Marcel Schaaf
Universitair hoofddocent
- Naam
- Dr. M.J.M. Schaaf
- Telefoon
- +31 71 527 4975
- m.j.m.schaaf@biology.leidenuniv.nl
- ORCID iD
- 0000-0002-5859-8936
Voor meer informatie, zie de Engelse versie.
Promovendi
Postdocs
Nieuws
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Dit is wat je als student wilt weten van de nieuwe opleidingsdirecteuren Biologie -
Twee nieuwe opleidingsdirecteuren bij het Instituut Biologie Leiden -
Marcel Schaaf: 'Docenten moeten van hun eilandjes af' -
Maar liefst vier W&N-docenten behalen SKO-certificaat -
Gratis online cursus leert je alles over imaging -
Miljoenensubsidie om biologische klok gezond te houden -
Digitaal sprinkhanen bekijken en microscopie op afstand -
Plastic nanodeeltjes maken zebravislarven hyperactief -
Proactieve persoonlijkheid heeft sterker waak-slaapritme -
Profileringsprogramma over lichaamseigen marihuana brengt instituten bij elkaar -
Europees project ImageInLife van start gegaan -
Het effect van ontstekingsremmende medicijnen op ons lichaam ten aanzien van stress
Oud-promovendi
Universitair hoofddocent
- Wiskunde en Natuurwetenschappen
- Instituut Biologie Leiden
- IBL Animal Sciences
- Mever M. van, Mamani-Huanca M., Faught L.E., Lopez-Gonzalvez A., Hankemeier T., Barbas C., Schaaf M.J.M. & Ramautar R. (2024), Application of a capillary electrophoresis-mass spectrometry metabolomics workflow in zebrafish larvae reveals new effects of cortisol, Electrophoresis 45(5-6): 380-391.
- Radoslaw J., Jong B. de, Hage P. van, Rhiemus M., Steenis F. van, Noort S.J.T. van, Schmidt T. & Schaaf M.J.M (2022), Analysis of the H-Ras mobility pattern in vivo shows cellular heterogeneity inside epidermal tissue, Disease Models & Mechanisms 15(2): dmm049099.
- Xie Y., Papadopoulou P., Wit B. de, D'Engelbronner J.C., Hage P. van, Kros A. & Schaaf M.J.M. (2022), Two types of liposomal formulations improve the therapeutic ratio of prednisolone phosphate in a zebrafish model for inflammation, Cells 11(4): 671.
- Rock S., Rodenburg F.J., Schaaf M.J.M. & Tudorache C. (2022), Detailed analysis of zebrafish larval behaviour in the light dark challenge assay shows that diel hatching time determines individual variation, Frontiers in Physiology 13: 827282.
- Schaaf M.J.M. & Meijer O.C. (2022), Immune modulations by glucocorticoids: from molecular biology to clinical research, Cells 11(24): 4032.
- Xiong Y., Halima M., Che X.Y., Zhang Y.M., Schaaf M.J.M., Li M.H., Gao M., Guo L.Q., Huang Y., Cui X.M. & Wang M. (2022), Steamed panax notoginseng and its saponins inhibit the migration and induce the apoptosis of neutrophils in a zebrafish tail-fin amputation model, Frontiers in Pharmacology 13: 946900.
- Davis R., Luchtenburg F., Richardson M.K., Schaaf M.J.M., Tudorache C. & Slabbekoorn H.W. (2021), The importance of individual variation for the interpretation of behavioural studies: ethanol effects vary with basal activity level in zebrafish larvae, Psychopharmacology 238: 3155-3166.
- Xie Y., Xie J., Meijer A.H. & Schaaf M.J.M. (2021), Glucocorticoid-induced exacerbation of mycobacterial infection is associated with a reduced phagocytic capacity of macrophages, Frontiers in Immunology 12: 618569.
- Hosseini R., Lamers G.E.M., Bos E., Hogendoorn P.C.W., Koster A.J., Meijer A.H., Spaink H.P. & Schaaf M.J.M. (2021), The adapter protein Myd88 plays an important role in limiting mycobacterial growth in a zebrafish model for tuberculosis, Virchows Archiv 479(2): 265-275.
- Xie Y., Meijer A.H. & Schaaf M.J.M. (2021), Modeling inflammation in zebrafish for the development of anti-inflammatory drugs, Frontiers in Cell and Developmental Biology 8: 620984.
- Bernardello M., Gora R.J., Hage P. van, Castro-Olvera G., Gualda E.J., Schaaf M.J.M. & Loza-Alvarez P. (2021), Analysis of intracellular protein dynamics in living zebrafish embryos using light-sheet fluorescence single-molecule microscopy, Biomedical Optics Express 12(10): 6205-6227.
- He M., Halima M., Xie Y., Schaaf M.J.M., Meijer A.H. & Wang M. (2020), Ginsenoside Rg1 acts as a selective glucocorticoid receptor agonist with anti-inflammatory action without affecting tissue regeneration in zebrafish larvae , Cells 9(5): 1107.
- Puyskens A., Stinn A., Vaart M. van der, Kreuchwig A., Protze J., Pei G., Klemm M., Guhlich-Bornhof U., Hurwitz R., Krishnamoorthy G., Schaaf M.J.M., Krause G., Meijer A.H., Kaufmann S.H.E. & Moura-Alves P. (2020), Aryl hydrocarbon receptor modulation by tuberculosis drugs impairs host defense and treatment outcomes , Cell Host and Microbe 27(2): 238-248.e7.
- Bos R. van den, Cromwijk S., Tschigg K., Althuizen J., Zethof J., Whelan R., Flik G. & Schaaf M.J.M. (2020), Early life glucocorticoid exposure modulates immune function in zebrafish (Danio rerio) larvae, Frontiers in Immunology 11: 727.
- Jaikumar G., Slabbekoorn H.W., Sireeni J., Schaaf M.J.M. & Tudorache C. (2020), The role of the glucocorticoid receptor in the regulation of diel rhythmicity, Physiology and Behavior 223: 112991.
- Sireeni J., Bakker N., Jaikumar G., Obdam D., Slabbekoorn H.W., Tudorache C. & Schaaf M.J.M. (2020), Profound effects of glucocorticoid resistance on anxiety-related behavior in zebrafish adults but not in larvae, General and Comparative Endocrinology 292: 113461.
- Xie Y., Tolmeijer S., Oskam J.M., Tonkens T., Meijer A.H. & Schaaf M.J.M. (2019), Glucocorticoids inhibit macrophage differentiation towards a pro-inflammatory phenotype upon wounding without affecting their migration, Disease Models & Mechanisms 12(5): 037887.
- Brun N.R., Hage P. van, Hunting E.R., Haramis A.-P.G., Vink S.C., Vijver M.G., Schaaf M.J.M. & Tudorache C. (2019), Polystyrene nanoplastics disrupt glucose metabolism and cortisol levels with a possible link to behavioural changes in larval zebrafish, Communications Biology 2: 382.
- Keizer V.I.P., Coppola S., Houtsmuller A.B., Geverts B., Royen M.E. van, Schmidt T. & Schaaf M.J.M. (2019), Repetitive switching between DNA-binding modes enables target finding by the glucocorticoid receptor, Journal of Cell Science 132(5): jcs217445.
- Luchtenburg F.J., Schaaf M.J.M. & Richardson M.K. (2019), Functional characterization of the cannabinoid receptors 1 and 2 in zebrafish larvae using behavioral analysis, Psychopharmacology 236(7): 2049-2058.
- Palstra A.P., Mendez S., Dirks R.P. & Schaaf M.J.M. (2019), Cortisol acting through the glucocorticoid receptor is not involved in exercise-enhanced growth, but does affect the white skeletal muscle transcriptome in zebrafish (danio rerio), Frontiers in Physiology 9: 1889.
- Meijer O.C., Buurstede J.C. & Schaaf M.J.M. (2018), Corticosteroid Receptors in the Brain: Transcriptional Mechanisms for Specificity and Context-Dependent Effects, Cellular and Molecular Neurobiology 39(4): 539-549.
- Gissendanner C.R., Baldwin W.S. & Schaaf M.J.M. (2018), Non-Mammalian Nuclear Receptors: From Evolution to Human Disease, Nuclear Receptor Research 5: 101366.
- Tudorache C., Slabbekoorn H.W., Robbers Y., Hin E., Meijer A.H., Spaink H.P. & Schaaf M.J.M. (2018), Biological clock function is linked to proactive and reactive personality types, BMC Biology 16: 148.
- Chatzopoulou A., Schoonheim P.J., Torraca V., Meijer A.H., Spaink H.P. & Schaaf M.J.M. (2017), Functional analysis reveals no transcriptional role for glucocorticoid receptor beta-isoform in zebrafish, Molecular and Cellular Endocrinology 447: 61-70.
- Schaaf M.J.M. (2017), Nuclear receptor research in zebrafish, Journal of Molecular Endocrinology 59(1): R65-R76.
- Schaaf M.J.M. (2017), The First Fifteen Years of Steroid Receptor Research in Zebrafish; Characterization and Functional Analysis of the Receptors, Nuclear Receptor Research 4: 101286.
- Amin B., Slabbekoorn H., Schaaf M.J.M. & Tudorache C. (2016), "Early birds" take it easy: diurnal timing is correlated with overall level in activity of zebrafish larvae, Behaviour 153(13-14): 1745-1762.
- Carattino A.J., Keizer V.I.P., Schaaf M.J.M. & Orrit M.A.G.J. (2016), Background suppression in imaging gold nanorods through detection of anti-Stokes emission, Biophysical Journal 111(11): 2492-2499.
- Chatzopoulou Chatzi A., Heijmans J.P., Burgerhout E., Oskam N., Spaink H.P., Meijer A.H. & Schaaf M.J.M. (2016), Glucocorticoid-Induced Attenuation of the Inflammatory Response in Zebrafish, Endocrinology 157(7): 2772-2784.
- Hosseini R., Lamers G.E.M., Soltani H.M., Meijer A.H., Spaink H.P. & Schaaf M.J.M. (2016), Efferocytosis and extrusion of leukocytes determine the progression of early mycobacterial pathogenesis, Journal of Cell Science 129(18): 3385-3395.
- Tudorache C., Braake A. ter, Tromp M., Slabbekoorn H.W. & Schaaf M.J.M. (2015), Behavioral and physiological indicators of stress coping styles in larval zebrafish, Stress 18(1): 121-128.
- Neo Y.Y., Parie L., Bakker F., Snelderwaard P.C., Tudorache C., Schaaf M.J.M. & Slabbekoorn H.W. (2015), Behavioral changes in response to sound exposure and no spatial avoidance of noisy conditions in captive zebrafish, Frontiers in Behavioral Neuroscience 9: 28.
- Harkes R., Keizer V.I.P., Schaaf M.J.M. & Schmidt T. (2015), Depth-of-Focus Correction in Single-Molecule Data Allows Analysis of 3D Diffusion of the Glucocorticoid Receptor in the Nucleus, PLoS ONE 10(11): e0141080.
- Geurtsen A., Lamers Maarten H. & Schaaf M.J.M. (2015), Interactive Digital Gameplay Can Lower Stress Hormone Levels in Home Alone Dogs — A Case for Animal Welfare Informatics. Chorianopoulos K., Divitini M., Baalsrud Hauge J., Jaccheri L. & Malaka R. (red.), Entertainment Computing - ICEC 2015 (Lecture Notes in Computer Science Vol. 9353). 14th International Conference, ICEC 2015 29 september 2015 - 2 oktober 2015. Trondhei,: Springer International Publishing. 238-251.
- Chatzopoulou Chatzi A., Upasana R., Meijer A.H., Alia A., Spaink H.P. & Schaaf M.J.M. (2015), Transcriptional and metabolic effects of glucocorticoid receptor alpha and beta signaling in zebrafish, Endocrinology 156(5): 1757-1769.
- Royen M.E. van, Cappellen W.A. van, Geverts B., Schmidt T., Houtsmuller A.B. & Schaaf M.J.M. (2014), Androgen receptor complexes probe DNA for recognition sequences by short random interactions, Journal of Cell Science 127(7): 1406-1416.
- Groeneweg F.L., Royen M.E. van, Fenz F.S., Keizer V.I.P., Geverts B., Prins J., Kloet E.R. de, Houtsmuller A.B., Schmidt T. & Schaaf M.J.M. (2014), Quantitation of Glucocorticoid Receptor DNA-Binding Dynamics by Single-Molecule Microscopy and FRAP, PLoS ONE 9(3): 90532.
- Hosseini R., Lamers G.E.M., Hodzic Z., Meijer A.H., Schaaf M.J.M. & Spaink H.P. (2014), Correlative light and electron microscopy imaging of autophagy in a zebrafish infection model, Autophagy 10(10): 1844-1857.
- Tudorache C, Ter Braake A, Tromp M, Slabbekoorn H & Schaaf MJ. (2014), Behavioural and physiological indicators of stress coping styles in larval zebrafish, Stress 19(Nov): 1-23.
- Tudorache C., Schaaf M.J.M. & Slabbekoorn H.W. (2013), Covariation between behaviour and physiology indicators of coping style in zebrafish (Danio rerio), Journal of Endocrinology 219(3): 251-258.
- Palstra A.P., Schaaf M.J.M. & Planas J.V. (2013), Exercise physiology of zebrafish: Swimming effects on skeletal and cardiac muscle growth, on the immune system, and the involvement of the stress axis. In: Palstra A. & Planas J. (red.), Swimming physiology of fish: Towards using exercise to farm a fit fish in sustainable aquaculture.: Springer-Verlag Berlin Heidelberg. 323-342.
- Kanwal Z., Zakrzewska A., Den Hertog J., Spaink H.P., Schaaf M.J. & Meijer A.H. (2013), Deficiency in hematopoietic phosphatase Ptpn6/Shp1 hyperactivates the innate immune system and impairs control of bacterial infections in zebrafish embryos, Journal of Immunology 190(4): 1631-1645.
- Raterink R., Kloet F.M. van der, Li J., Wattel N.A., Schaaf M.J.M., Spaink H.P., Berger R., Vreeken R.J. & Hankemeier T. (2013), Rapid metabolic screening of early zebrafish embryogenesis based on direct infusion-nanoESI-FTMS, Metabolomics 9: 864–873.
- Porta F., Lamers G.E., Morrhayim G., Chatzopoulou A., Schaaf M.J.M., Dulk H. den, Backendorf C., Zink J.I. & Kros A. (2013), Folic acid modified mesoporous silica nanoparticles for cellular and nuclear targeted drug delivery, Advanced Healthcare Materials 2(2): 281-286.
- Meckel T., Semrau S., Schaaf M.J.M. & Schmidt T. (2012), Robust assessment of protein complex formation in vivo via single-molecule intensity distributions of autofluorescent proteins, Journal of Biomedical Optics 16: 76016.
- Ziv L., Muto A., Schoonheim P.J., Meijsing S.H., Strasser D., Ingraham H.A., Schaaf M.J.M., Yamamoto K.R. & Baier H. (2012), An affective disorder in zebrafish with mutation of the glucocorticoid receptor, Molecular Psychiatry 18: 681-691.
- Meckel T.M., Semrau S., Schaaf M.J.M. & Schmidt T. (2011), Robust assesment of protein complex formation in vivo via single molecule intensity distibutions of autofluorescent proteins, Journal for Biomedical Optics 16(076016): .
- Schaaf M.J.M. & Schmidt T. (2011), In Vivo Single-Molecule Microscopy Using the Zebrafish Model System. In: Sako Y. & Ueda M. (red.), Cell Signaling Reactions: Single-Molecular Kinetic Analyses.
- Schoonheim P.J., Chatzopoulou A. & Schaaf M.J.M. (2010), The zebrafish as an in vivo model system for glucocorticoid resistance, Steroids 75(12): 918-925.
- Vaart M. van der & Schaaf M.J.M. (2009), Naturally occurring C-terminal splice variants of nuclear receptors, Nucl. Recept. Signal. 7: e007.
- Sarabdjitsingh R.A., Meijer O.C., Schaaf M.J.M. & Kloet E.R. de (2009), Subregion-specific differences in translocation patterns of mineralocorticoid and glucocorticoid receptors in rat hippocampus, Brain Res. 1249: 43-53.
- Schaaf M.J.M., Koopmans W., Meckel T.M., Noort S.J.T. van, Snaar B.E., Schmidt T. & Spaink H.P. (2009), Single-molecule microscopy reveals membrane microdomain organization of cells in a living vertebrate, Biophysical Journal 97(4): 1206-1214.
- Schaaf M.J.M., Chatzopoulou Chatzi A. & Spaink H.P. (2009), The zebrafish as a model system for glucocorticoid receptor research, Comparative Biochemistry and Physiology. A. Molecular Integrative Physiology 153(1): 75-82.
- Brittijn S.A., Duivesteijn S.J., Belmamoune M., Bertens L.M.F., Bitter W., Bruijn J.D. de, Champagne D.L., Cuppen E., Flik G., Broucke-Grauls C.M. van den, Janssen R., Jong I.E.M. de, Kloet E.R. de, Kros A., Meijer A., Metz J.R., Sar A.M. van der, Schaaf M.J., Schulte-Merker S., Spaink H.P., Tak P.P., Verbeek F.J., Vervoordeldonk M.J., Vonk F., Witte F., Yuan H. & Richardson M.K. (2009), Zebrafish development and regeneration: new tools for biomedical research, The International Journal of Developmental Biology 53(5-6): 835-850.
- Fitzsimons C.P., Ahmed S., Wittevrongel C., Schouten T.G., Dijkmans T.F., Scheenen W.J., Schaaf M.J.M., Kloet E.R. de & Vreugdenhil E. (2008), The microtubule associated protein Doublecortin-like regulates the transport of the glucocorticoid receptor in neuronal progenitor cells, Molecular Endocrinology 22: 248-262.
- Schaaf M.J.M., Champagne D., Laanen I.H. van, Wijk D.C. van, Meijer A.H., Meijer O.C., Spaink H.P. & Richardson M.K. (2008), Discovery of a functional glucocorticoid receptor {beta}-isoform in zebrafish, Endocrinology 149(4): 1591-1599.
- Zhu Y., Hanna R.N., Schaaf M.J.M., Spaink H.P. & Thomas P. (2008), Candidates for membrane progestin receptors: past approaches and future challenges, Comparative Biochemistry Physiology C Toxicology Pharmacology 148(4): 381-389.
- Vreugdenhil E., Fitzsimons C.P., Kolk S.M., Schaaf M.J.M. & Lucassen P.J. (2007), Doublecortin-Like, a Microtubule Associated Protein Expressed in Radial Glia is Crucial for Neuronal Precursor Division and Radial Process Stability, European Journal of Neuroscience 25: 635-648.
- Schaaf M.J.J., Willetts L., Hayes B.P., Maschera B., Stylianou E. & Farrow S.N. (2006), The relationship between intranuclear mobility of the NF-kappa B subunit p65 and its DNA binding affinity, Journal of Biological Chemistry 281(31): 22409-22420.
- Schaaf M.J., Lewis-Tuffin L.J. & Cidlowski J.A. (2005), Ligand-selective targeting of the glucocorticoid receptor to neclear subdomains is associated with decreased receptor mobility, Molecular Endocrinology 19(6): 1501-1515.
- Schaaf M.J. & Cidlowski J.A. (2003), Molecular determinants of glucocorticoïd receptor mobility in living cells: the importance of ligand affinity, Molecular and Cellular Biology 23(6): 1922-1934.
- Rijk R.H. de, Schaaf M.J.M., Stam F.J., Jong I.E.M., Swaab D.F., Ravid R., Vreugdenhil E., Kloet E.R. de & Lucassen P.J. (2003), Very low levels of the glucocorticoid receptor B isoform in the human hippocampus as shown by Tagman RT-PCR and immunocytochemistry, Molecular Brain Research 116: 17-26.
- Rijk R.H. de, Schaaf M.J.M., Stam F.J., Jong I.E.M., Swaab D.F., Ravid R., Vreugdenhil E., Cidlowski J.A., Kloet E.R. de & Lucassen P.J. (2003), Very low levels of the glucocorticoid receptor beta-isoform in the human hippocampus as shown by Taqman RT-PCR and immunocytochemistry, Molecular Brain Research 116: 17-26.
- Rijk R.H. de, Schaaf M.J.M., Stam F.J., Jong I.E.M., Swaab D.F., Ravid R., Vreugdenhil E., Cidlowski J.A., Kloet E.R. de & Lucassen P.J. (2003), Very low levels of the glucocorticoid receptor beta isoform in the human hippocampus as shown by Taqman RT-PCR and immunocytochemistry, Molecular Brain Research 116: 17-26.
- Schaaf M.J. & Cidlowski J.A. (2002), The glucocorticoïd receptor beta-isoform: a perspective on its relevance in human health and disease (Ernst Schering Res Found). [overig].
- Schaaf M.J.M. & Cidlowski J.A. (2002), AUUUA motifs in the 3'UTR of human glucocorticoïd receptor alpha and beta mRNA destabilize mRNA and decrease receptor protein expression, Steroids 67(7): 627-636.
- Rijk R.H. de, Schaaf M.J.M. & Kloet E.R. de (2002), Glucocorticoid receptor variants: clinical implications, Journal of Steroid Biochemistry & Molecular Biology 81: 103-122.
- Vreugdenhil E., Kloet E.R. de, Schaaf M.J.M. & Datson N.A. (2001), Genetic dissection of corticosterone receptor function in the rat hippocampus, European Neuropsychopharmacology 11(6): 423-430.
- Rijk R.H. de, Schaaf M.J.M., Turner G., Datson N.A., Vreugdenhil E., Cidlowski J., Kloet E.R. de, Emery P., Sternberg E.M. & Detera-Wadleigh S.D. (2001), A human glucocorticoid receptor gene variant that increases the stability of the glucocorticoid receptor betaisoform mRNA is associated with rheumatoid arthritis, Journal of Rheumatology 28: 2383-2388.
- Schaaf M.J.M., Workel J.O., Lesscher H., Vreugdenhil E., Oitzl M.S. & Kloet E.R. de (2001), Correlation between hippocampal BDNF mRNA expression and memory performance in senescent rats, Brain Research 915: 227-233.
- Schaaf M.J.M., Kloet E.R. de & Vreugdenhil E. (2000), Corticosterone effects on BDNF expression in the hippocampus (Review), Stress 3: 201-208.
- Schaaf M.J.M., Duurland R., Kloet E.R. de & Vreugdenhil E. (2000), Circadian variation in BDNF mRNA expression in the rat hipocampus, Molecular Brain Research 75(2): 342-344.
- Schaaf M.J., Sibug R.M., Duurland R., Fluttert M.F., Oitzl M.S., Kloet E.R. de & Vreugdenhil E. (1999), Corticosterone effects on BDNF mRNA expression in the rat hippocampus during morris water maze training, Stress 3(2): 173-183.
- Vreugdenhil E., Datson N., Engels B., Jong J. de, Koningsbruggen S. van, Schaaf M.J.M. & Kloet E.R. de (1999), Kainate-elicited seizures induce mRNA encoding a CaMK-related peptide: a putative modulator of kinase activity in rat hippocampus, Journal of Neurobiology 39(1): 41-50.
- Schaaf M.J.M., Jong J. de, Kloet E.R. de & Vreugdenhil E. (1998), Downregulation of BDNF mRNA and protein in the rat hippocampus by corticosterone, Brain Research Bulletin 813: 112-120.
- Schaaf M.J.M., Hoetelmans R.W.M., Kloet E.R. de & Vreugdenhil E. (1997), Corticosterone regulates expression of BDNF and trkB but not NT-3 and trkC mRNA in the rat hippocampus, Journal of Neuroscience Research 48: 334-341.
- Vreugdenhil E., Jong J. de, Schaaf M.J., Meijer O.C., Busscher J., Vuijst C. & Kloet E.R. de (1996), Molecular dissection of corticosteroid action in the rat hippocampus. Application of the differential display techniques, Journal of Molecular Neuroscience 7(2): 135-146.