Ilze Bot
Professor Cardiovascular pharmacology
- Name
- Prof.dr. I. Bot
- Telephone
- +31 71 527 5682
- i.bot@lacdr.leidenuniv.nl
- ORCID iD
- 0000-0002-1242-1959
Prof. dr. Ilze Bot is Professor of Cardiovascular Pharmacology at the Division of BioTherapeutics at the Leiden Academic Centre for Drug Research (LACDR, Leiden University). Her research programme is focused on the elucidation of the molecular and cellular mechanisms that underlie atherosclerotic plaque destabilization, thereby identifying novel drug targets and candidates. She is an Established Investigator of the Dutch Heart Foundation.
More information about Ilze Bot
PhD Candidates
News
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Arteriosclerosis and drug discovery: two young researchers win Krijn Rietveld Award for innovative research -
Atherosclerosis resembles an autoimmune disease. Marie Depuydt graduated on this topic, with honours -
Ilze Bot appointed professor of Cardiovascular Pharmacology -
Preventing heart attacks by earlier detection of cardiovascular disease -
EIC Pathfinder Challenge grant for research into autoreactive B cells in cardiovascular disease -
In conversation with the head of the rodent facility -
Website full of interactive videos to improve your lab education -
Why you should publish negative data -
Why arteriosclerosis looks like an autoimmune disease -
Improving education with videos and humour -
Ilze Bot receives prestigious grant from the Dutch Heart Foundation -
Portraits Johan Kuiper and Ilze Bot on Hartstichting.nl
Ilze Bot obtained her PhD on “Modulation of Atherothrombotic Factors: Novel Strategies for Plaque Stabilization” under supervision of Prof. Dr. E.A.L. Biessen and Prof. Dr. Th. J.C. van Berkel in 2005. In March 2008 she started working as a Post-doctoral researcher on mast cells and atherosclerosis (VENI grant “Cardiovascular Diseases: A Central Role for Mast Cells?”) at the division of Biopharmaceutics of the Leiden Academic Centre for Drug Research. Since then, she expanded her research line on mast cells in cardiovascular diseases by obtaining two research grants from the Dutch Heart Foundation to investigate the contribution of the mast cell to stress-induced acute cardiovascular syndromes, and effects of mast cell activation in vein graft disease. In 2012, she was awarded a Dr. E. Dekker senior Postdoc grant from the Dutch Heart Foundation to determine how mast cells can be activated during hypercholesterolemia. In December 2019, Ilze received the Established Investigator grant from the Dutch Heart Foundation for her project "Mast cells as effectors in advanced atherosclerosis". She focusses her research on mast cell recruitment and activation pathways and on the characterization of mast cells phenotypes in atherosclerosis. In addition, she coordinates the ERA4Health CARDINNOV program “MASTer”, in which she investigates the contribution of mast cells to intraplaque hemorrhage, a hallmark of unstable atherosclerosis. She has published her research in high impact journal such as Circulation, Circulation Research, Nature Cardiovascular Research and Annals of Surgery, and frequently invited to give lectures at international conferences.
As of March 1st 2024, Ilze is appointed as full professor in Cardiovascular Pharmacology at Leiden University. She will continue her work elucidating the underlying mechanisms of atherosclerotic plaque destabilization at the division of BioTherapeutics of the Leiden Academic Centre for Drug Research.
Project: Mast cells in cardiovascular diseases
Pathology studies have shown that the mast cell, a potent immune effector cell type mainly recognized for its role in allergy and asthma, accumulates in the atherosclerotic plaque during atherosclerotic plaque progression. Previously, we have demonstrated that perivascular mast cells contribute to atherosclerotic plaque progression and destabilization and recent data show that intraplaque mast cell numbers are of predictive value for future cardiovascular events in patients. In this research line, we aim to determine how mast cells contribute to the development and progression of atherosclerosis and how these cells are activated during the disease process. Using these mechanistic insights, we aim to establish how we can prevent atherosclerotic plaque destabilization by targeting the mast cell.
Professor Cardiovascular pharmacology
- Science
- Leiden Academic Centre for Drug Research
- LACDR/Biopharmaceutics
- Elieh-Ali-Komi D., Bot I., Rodríguez-González M. & Maurer M. (2024), Cellular and molecular mechanisms of mast cells in atherosclerotic plaque progression and destabilization, Clinical Reviews in Allergy and Immunology 66: 30-49.
- Mulholland M., Depuydt M.A.C., Jakobsson G., Ljungcrantz I., Grentzmann A., To F., Bengtsson E., Jaensson Gyllenbäck E., Grönberg C., Rattik S., Liberg D., Schiopu A., Björkbacka H., Kuiper J., Bot I., Slütter B.A. & Engelbertsen D. (2024), Interleukin-1 receptor accessory protein blockade limits the development of atherosclerosis and reduces plaque inflammation, Cardiovascular Research 120(6): 581-595.
- Jong M.J.M. de, Schaftenaar F.H., Depuydt M.A.C., Lozano Vigario F., Janssen G.M.C., Peeters J.A.H.M., Goncalves L., Wezel A., Smeets H.J., Kuiper J., Bot I., Veelen P. van & Slütter B.A. (2024), Virus-associated CD8+T-cells are not activated through antigen-mediated interaction inside atherosclerotic lesions, Arteriosclerosis, Thrombosis, and Vascular Biology 44: 1302-1314.
- Schaftenaar F.H., Dam A.D. van, Bruin G. de, Depuydt M.A.C., Mol J., Amersfoort J., Douna H., Meijer M., Kröner M.J., Santbrink P.J. van, Bernabé Kleijn M.N.A., Puijvelde G.H.M. van, Florea B.I., Slütter B., Foks A.C., Bot I., Rensen P.C.N. & Kuiper J. (2024), Immunoproteasomal inhibition with ONX-0914 attenuates atherosclerosis and reduces white adipose tissue mass and metabolic syndrome in mice, Arteriosclerosis, Thrombosis, and Vascular Biology 44(6): 1346–1364.
- Jong M.J.M. de, Depuydt M.A.C., Schaftenaar F.H., Liu K., Maters D., Wezel A., Smeets H.J., Kuiper J., Bot I., Gisbergen K. van & Slütter B.A. (2024), Resident memory T cells in the atherosclerotic lesion associate with reduced macrophage content and increased lesion stability, Arteriosclerosis, Thrombosis, and Vascular Biology 44(6): 1318–1329.
- Singh A., Kraaijeveld A.O., Curaj A., Wichapong K., Hammerich L., Jager S.C.A. de, Bot I., Atamas S.P., Berkel T.J.C. van, Jukema J.W., Comerford I., McColl S.R., Mees B., Heemskerk J.W.M., Nicolaes G.A.F., Hackeng T., LiehnE.A., Tacke F. & Biessen E.A.L. (2024), CCL18 aggravates atherosclerosis by inducing CCR6-dependent T-cell influx and polarization, Frontiers in Immunology 15: 1327051.
- Smit V., Mol J. de, Bernabé Kleijn M.N.A.B., Depuydt M.A.C., Winther M.P.J. de Bot I., Kuiper J. & Foks A.C. (2024), Sexual dimorphism in atherosclerotic plaques of aged Ldlr-/- mice, Immunity and Ageing 21(1): 27.
- Depuydt M.A.C., Smit V., Schaftenaar F.H., Hemme E., Delfos L., Vlaswinkel F.D., Simpson C., Kovanen P.T., Slütter B.A., Kuiper J., Bot I. & Foks A.C. (2024), Aging promotes mast cell activation and antigen presenting capacity in atherosclerosis, Cardiovascular Research 120(Supplement 1): cvae088.201.
- Prange K., Bel-Bordes G., Depuydt M.A.C., Kempkes R.W.M., Jong M.D.M. de, Mol B.M., Slütter B., Bot I., Neele A.E., Kleijn D.P.V. de, Borst G.J. de, Kuiper J., Pasterkamp. G., Mokry M. & Winther M.P.J. de (2024), Human atherosclerotic plaque macrophages associate with clinical presentation and follow diverse lineage differentiation routes, Cardiovascular Research 120(Supplement 1): cvae088.168.
- Medina Rodriguez Indira, Wieland E.B., Temmerman L., Otten J.J.T., Bermudez B., Bot I., Rademakers T., WijnandsE., Schurgers L., Mees B., Berkel T.J.C. van, Goossens P. & Biessen E.A.L. (2024), Colony stimulating factor 1 receptor (Csf1r) expressing cell ablation in mafia (macrophage-specific Fas-induced apoptosis) mice alters monocyte landscape and atherosclerotic lesion characteristics, European Journal of Immunology 54(11): 2350943.
- Bot I., Delfos L., Hemme E., Bernabé Kleijn M.N.A., Santbrink P.J. van, Foks A.C., Kovanen P.T., Jouppila A. & Lassila R. (2024), Treatment with APAC, a dual antiplatelet anticoagulant heparin proteoglycan mimetic, limits early collar-induced carotid atherosclerotic plaque development in Apoe-/- mice, Atherosclerosis 397: 118567.
- Hemme E., Depuydt M.A.C., Santbrink P.J. van, Wezel A., Smeets H.J., Foks A.C., Kuiper J. & Bot I. (2024), Leukemia inhibitory factor receptor inhibition by EC359 reduces atherosclerotic stenosis grade in Ldlr-/- mice, European Journal of Pharmacology 985: 177121.
- Panhuis W. in het, Schönke M., Modder M., Tom H.E., Lalai R.A., Pronk A.C.M., Streefland T.C.M., Kerkhof L.W.M. van, Dollé M.E.T., Depuydt M.A.C., Bot I., Vos W.G., Bosmans L.A., Os B.W. van, Lutgens E., Rensen P.C.N. & Kooijman S. (2023), Time-restricted feeding attenuates hypercholesterolaemia and atherosclerosis development during circadian disturbance in APOE∗3-Leiden.CETP mice, EBioMedicine 93: 104680.
- Smit V., Mol J. de, Schaftenaar F.H., Depuydt M.A.C., Postel R.J., Smeets D.: Verheijen F.W.M., Bogers L., Duijn J. van, Verwilligen R.A.F., Grievink H.W., Bernabé Kleijn M.N.A., Ingen E. van, Jong M.J.M. de, Goncalves L.: Peeters J.A.H.M., Smeets H.J., Wezel A., Polansky J.K., Winther M.P.J. de, Binder C.J., Tsiantoulas D., Bot I., Kuiper J. & Foks A.C. (2023), Single-cell profiling reveals age-associated immunity in atherosclerosis, Cardiovascular Research 119(15): 2508-2521.
- Lozano Vigario F., Simo Vesperinas I., Depuydt M.A.C., Bot I., Veelen P. van, Bouwstra J.A., Kros A. & Slutter B. (2023), Immunopeptidomic analysis of human atherosclerosis identifies novel ApoB100-derived antigenic drivers of atherosclerosis, Atherosclerosis 379: S8-S9.
- Hemme E., Depuydt M.A.C., Delfos L., Kuiper J. & Bot I. (2023), Leukemia inhibitory factor receptor inhibition in atherosclerosis, Atherosclerosis 379: S36.
- Delfos L., Depuydt M.A.C., Foks A.C., Bernabe Kleijn M.N.A., Kuiper J., Chemaly M., Peace A., McGilligan V. & Bot I. (2023), NLRP3 inflammasome inhibition by the novel bispecific antibody inflamab inhibits atherosclerosis in apolipoprotein E-deficient mice, Atherosclerosis 379(Supplement 1): S2.
- Segers F.M.E., Ruder A.V., Westra M.M., Lammers T., Dadfar S.M., Roemhild K., Lam T.S., Kooi M.E., Cleutjens K.B.J.M., Verheyen F.K., Schurink G.W.H., Haenen, G.W.H., Berkel T.J.C. van, Bot I., Halvorsen B., Sluimer J.C. & Biessen E.A.L. (2023), Magnetic resonance imaging contrast-enhancement with superparamagnetic iron oxide nanoparticles amplifies macrophage foam cell apoptosis in human and murine atherosclerosis, Cardiovascular Research 118(17): 3346-3359.
- Depuydt M.A.C., Schaftenaar F.H., Prange H.H.M., Boltjes A., Hemme E., Delfos L., Mol J. de, Jong M.J.M. de, Bernabé Kleijn M.N.A., Peeters J.A.H.M., Goncalves L., Wezel A., Smeets H.J., Borst G.J. de, Foks A.C., Pasterkamp G., Winther M.P.J. de, Kuiper J., Bot I. & Slütter B. (2023), Single-cell T cell receptor sequencing of paired human atherosclerotic plaques and blood reveals autoimmune-like features of expanded effector T cells, Nature Cardiovascular Research 2: 112-125.
- Skenteris N.T., Hemme E., Delfos L., Karadimou G., Karlöf E., Lengquist M., Kronqvist M., Zhang X., Maegdefessel L., Schurgers L.J., Arnardottir H., Biessen E.A.L. & Bot I. Matic L. (2023), Mast cells participate in smooth muscle cell reprogramming and atherosclerotic plaque calcification, Vascular Pharmacology 150: 107167.
- Hemme E.: Biskop D., Depuydt M.A.C., Smit V., Delfos L., Bernabé Kleijn M.N.A., Foks A.C., Kuiper J. & Bot I. (2023), Bruton's Tyrosine Kinase inhibition by Acalabrutinib does not affect early or advanced atherosclerotic plaque size and morphology in Ldlr-/- mice, Vascular Pharmacology 150: 107172.
- Velden J. van der, Asselbergs F.W., Bakkers J., Batkai S., Bertrand L., Bezzina C.R., Bot I., Brundel B., Carrier L., Chamuleau S., Ciccarelli M., Dawson D., Davidson S.M., Dendorfer A., Duncker D.J., Eschenhagen T., Fabritz L., Falcão-Pires I., Ferdinandy P., Giacca M., Girao H., Gollmann-Tepeköylü C., Gyongyosi M., Guzik T.J., Hamdani N., Heymans S., Hilfiker A., Hilfiker-Kleiner D., Hoekstra A.G., Hulot J.S., Kuster D.W.D., Laake L.W. van, Lecour S., Leiner T., Linke W.A., Lumens J., Lutgens E., Madonna R., Maegdefessel L., Mayr M., Meer P. van der, Passier R., Perbellini F., Perrino C., Pesce M., Priori S., Remme C.A., Rosenhahn B., Schotten U., Schulz R., Sipido K., Sluijter J.P.G., Steenbeek F. van, Steffens S., Terracciano C.M., Tocchetti C.G., Vlasman P., Yeung K.K., Zacchigna S., Zwaagman D. & Thum T. (2022), Animal models and animal-free innovations for cardiovascular research: current status and routes to be explored. Consensus document of the ESC working group on myocardial function and the ESC working group on cellular biology of the heart, Cardiovascular Research 118(15): 3016-3051.
- Douna H., Mol J. de, Amersfoort J., Schaftenaar F.H., Kiss M.G., Suur B.E., Kroner M.J., Binder C.J., Bot I., Puijvelde G.H.M. van, Kuiper J. & Foks A.C. (2022), IFNγ-stimulated B cells inhibit T follicular helper cells and protect against atherosclerosis, Frontiers in Cardiovascular Medicine 9: 781436.
- Mol J. de, Postel R.J., Smit V., Bernabé Kleijn M.N.A., Bot I., Kuiper J. & Foks A.C. (2022), B cell depletion skews cd4+t cell towards a pro-inflammatory phenotype in aged atherosclerotic mice, Cardiovascular Research 118(Supplement_1): cvac066.230.
- Hemme E., Delfos L., Depuydt M.A.C., Bernabé Kleijn M.N.A., Schaftenaar F.H., Foks A.C., Kuiper J. & Bot I. (2022), Brutons tyrosine kinase inhibition to suppress mast cell activation in atherosclerosis, Cardiovascular Research 118(Supplement_1): cvac066.227.
- Depuydt M.A.C., Smit V., Lozano Vigario F., Bernabé Kleijn M.N.A., Vries M.R. de, Quax P.H.A., Wezel A., Smeets H.J., Kuiper J., Foks A.C., Bot I. & Slütter B.A. (2022), Granzyme B+CD4+T cells associate with an unstable plaque phenotype in advanced human atherosclerosis, Cardiovascular Research 118(Supplement_1): cvac066.229.
- Grievink H.W., Smit V., Huisman B.W., Gal P., Yavuz Y., Klerks C., Binder C.J., Bot I., Kuiper J., Foks A.C. & Moerland M. (2022), Cardiovascular risk factors: the effects of ageing and smoking on the immune system, an observational clinical study, Frontiers in immunology 13: 968815.
- Douna H., Smit V., Puijvelde G.H.M. van, Kiss M.G., Binder C.J., Bot I., Kuchroo V.K., Lichtman A.H., Kuiper J. & Foks A.C. (2022), Tim-1 mucin domain-mutant mice display exacerbated atherosclerosis, Atherosclerosis 352: 1-9.
- Bot I., Delfos L., Hemme E., Kovanen P.T., Jouppila A. & Lassila R. (2022), APAC treatment limits collar-induced carotid atherosclerotic plaque development in apoE-/- mice, Cardiovascular Research 118(Supplement_1): cvac066.183.
- Depuydt M.A.C., Vlaswinkel F.D., Hemme E., Delfos L., Bernabe Kleijn M.N.A., Santbrink P.J. van, Foks A.C., Slütter B., Kuiper J. & Bot I. (2022), Blockade of the BLT1-LTB4 axis does not affect mast cell migration towards advanced atherosclerotic lesions in LDLr-/- mice, Scientific Reports 12(1): 18362.
- Postel R.J., Smit V., De Mol J., Bernabé Kleijn M.N.A., De Jong M.J.M., Delfos L., Hemme E., Depuydt M.A.C., Bot I., Kuiper J. & Foks A.C. (2022), IL-21R blockade reduces atherosclerosis development in LDLR-/- mice, Atherosclerosis 355: 23.
- Amersfoort J., Schaftenaar F.H., Douna H., Santbrink P.J. van, Puijvelde G.H.M. van, Slütter B., Foks A.C., Harms A., Moreno-Gordaliza E., Wang Y., Hankemeier T., Bot I., Chi H. & Kuiper J. (2021), Diet-induced dyslipidemia induces metabolic and migratory adaptations in regulatory T cells, Cardiovascular Research 117(5): 1309-1324.
- Duijn J. van, Jong M.J.M. de, Benne N., Leboux R.J.T., Ooijen M.E. van, Kruit N., Foks A.C., Jiskoot W., Bot I., Kuiper J. & Slütter B. (2021), Tc17 CD8+ T-cells accumulate in murine atherosclerotic lesions, but do not contribute to early atherosclerosis development, Cardiovascular Research 117(14): 2755-2766.
- Bot I. (2021), The mast cell: a novel actor in cardiac microvessel dysfunction, Arteriosclerosis, Thrombosis, and Vascular Biology 41(4): 1337-1338.
- Mekke J.M., Egberts D.H.J., Waissi F., Timmerman N., Bot I., Kuiper J., Pasterkamp G., Borst G.J. de & Kleijn D.P.V. de (2021), Mast cell distribution in human carotid atherosclerotic plaque differs significantly by histological segment, European Journal of Vascular and Endovascular Surgery 62(5): 808-815.
- Grievink H.W., Smit V., Verwilligen R.A.F., Bernabé Kleijn M.N.A., Smeets D., Binder C.J., Yagita H., Moerland M., Kuiper J., Bot I. & Foks A.C. (2021), Stimulation of the PD-1 pathway decreases atherosclerotic lesion development in Ldlr deficient mice, Frontiers in Cardiovascular Medicine 8: 740531.
- Ingen E. van, Foks A.C.: Woudenberg T., Bent M.L. van der, Jong A. de, Hohensinner P.J., Wojta J., Bot I., Quax P.H.A. & Nossent A.Y. (2021), Inhibition of microRNA-494-3p activates Wntsignaling and reduces proinflammatorymacrophage polarization in atherosclerosis, Molecular Therapy - Nucleic Acids 26: 1228-1239.
- Douna H., Amersfoort J., Schaftenaar F.H., Kröner M.J., Kiss M.B., Slütter B.A., Depuydt M.A.C., Bernabé Kleijn M.N.A., Wezel A., Smeets H., Yagita H., Binder C.J., Bot I., Puijvelde G.H.M. van, Kuiper J. & Foks A.C. (2020), BTLA stimulation protects against atherosclerosis by regulating follicular B cells, Cardiovascular Research 116(2): 295-305.
- Ascone G., Di Ceglie I., Walgreen B., Sloetjes A.W., Lindhout E., Bot I., Loo F.A.J. van de, Koenders M.I., Kraan P.M. van der, Blom A.B., Bosch M.H.J. van den & Lent P.L.E.M. van (2020), High LDL levels lessen bone destruction during antigen-induced arthritis by inhibiting osteoclast formation and function, Bone 130: 115140.
- Bot I., Velden D. van der, Bouwman M., Kröner M.J., Kuiper J., Quax P.H.A. & Vries M.R. de (2020), Local Mast Cell Activation Promotes Neovascularization, Cells 9(3): 701.
- Depuydt M.A.C., Prange K.H.M., Slenders L., Örd T., Elbersen D., Boltjes A., Jager S.C.A. de, Asselbergs F.W., Borst G.J. de, Aavik E., Lönnberg T., Lutgens E., Glass C.K., Ruijter H.M. den, Kaikkonen M.U., Bot I., Slütter B., Laan S.W. van der, Yla-Herttuala S., Mokry M., Kuiper J., Winther M.P.J. de & Pasterkamp G. (2020), Microanatomy of the human atherosclerotic plaque by single-cell transcriptomics, Circulation Research 127(11): 1437-1455.
- Lagraauw H.M., Wezel A., Velden D. van der, Kuiper J. & Bot I. (2019), Stress-induced mast cell activation contributes to atherosclerotic plaque destabilization, Scientific Reports 9(1): 2134.
- Kritikou E., Depuydt M.A.C., Vries M.R. de, Mulder K.E., Govaert A.M., Smit M.D., Duijn J. van, Foks A.C., Wezel A., Smeets H.J., Slütter B., Quax P.H.A., Kuiper J. & Bot I. (2019), Flow Cytometry-Based Characterization of Mast Cells in Human Atherosclerosis, Cells 8(4): 334.
- Bot I., Mulder M.T., Indhirajanti S., Daele P.L.A. van & Roeters van Lennep J.E. (2019), Reply to: "The "cholesterol paradox" in patients with mastocytosis", Atherosclerosis 284: 262-263.
- Duijn J. van, Elsas M. van, Benne N., Depuydt M.A.C., Wezel A., Smeets H., Bot I., Jiskoot W., Kuiper J. & Slütter B.A. (2019), CD39 identifies a microenvironment-specific anti-inflammatory CD8+ T-cell population in atherosclerotic lesions, Atherosclerosis 285: 71-78.
- Douna H., Amersfoort J., Schaftenaar F.H., Kröner M.J., Kiss M.B., Slütter B., Depuydt M.A.C., Kleijn M.N.A.B., Wezel A., Smeets H., Yagita H., Binder C.J., Bot I., Puijvelde G.H.M. van, Kuiper J. & Foks A.C. (2019), B- and T-lymphocyte attenuator stimulation protects against atherosclerosis by regulating follicular B cells, Cardiovascular Research 116(2): 295-305.
- Hermans M., Roeters van Lennep J.E., Daele P. van & Bot I. (2019), Mast Cells in Cardiovascular Disease: From Bench to Bedside, International Journal of Molecular Sciences 20(14): 3395.
- Duijn J. van, Elsas M. van, Benne N., Depuydt M., Wezel A., Smeets H., Bot I., Jiskoot W., Kuiper J. & Slutter B. (2019), Continuous Tcr signaling in the atherosclerotic environment induces immunomodulatory Cd8+ T-cells expressing Cd39, Atherosclerosis 287: e17-e18.
- Laan S.W. van der, Slenders L., Depuydt M., Prange K., Granneman L., Elbersen D., Boltjes A., Jager S. de, Slutter B., Bot I., Winther M., Kuiper J., Mokry M., Asselbergs F. & Pasterkamp G. (2019), Mapping genes to cardiovascular susceptibility loci at a single-cell resolution, Atherosclerosis 287: E21-E21.
- Depuydt M., Prange K.H.M., Slenders L., Elbersen D., Boltjes A., De Jager S.C.A., Slutter B.A., Bot I., Laan S.W. van der, Mokry M., Pasterkamp G., Winther M.P.J. de & Kuiper J. (2019), Microanatomy of advanced human atherosclerotic plaques through single-cell transcriptomics, Atherosclerosis 287: E5-E5.
- Mokry M., Depuydt M.A.C., Prange K.H.M., Slenders L., Elbersen D., Granneman L.E.C., Jager S.C.A. de, Slutter B.A., Bot I., Winther M.P.J. de, Kuiper J., Asselbergs F.W., Laan S.W. van der & Pasterkamp G. (2019), Single cell Rna-sequencing identifies numerous cell sub-types and suggests lineage plasticity in human atherosclerotic plaques, Atherosclerosis 287: E96-E97.
- Douna H., Smit V., Puijvelde G. van, Binder C., Bot I., Kuchroo V., Lichtman A., Kuiper J. & Foks A. (2019), Tim-1 mucin domain-mutant mice display exacerbated atherosclerosis, Atherosclerosis 287: E25-E26.
- Ingen E. van, Foks A.C., Kröner M.J., Kuiper J., Quax P.H.A., Bot I. & Nossent Y. (2019), Inhibition Of Microrna-494 Halts Atherosclerotic Plaque Progression And Stabilizes Advanced Atherosclerotic Lesions, Atherosclerosis 287: E15.
- Ingen E. van, Foks A.C., Kröner M.J., Kuiper J., Quax P.H.A., Bot I. & Nossent A.Y. (2019), Antisense Oligonucleotide Inhibition of MicroRNA-494 Halts Atherosclerotic Plaque Progression and Promotes Plaque Stabilization, Molecular Therapy - Nucleic Acids 18: 638-649.
- Schaftenaar F.H., Amersfoort J., Douna H., Kröner M.J., Foks A.C., Bot I., Slütter B.A., Puijvelde G.H.M. van, Drijfhout J.W. & Kuiper J. (2019), Induction of HLA-A2 restricted CD8 T cell responses against ApoB100 peptides does not affect atherosclerosis in a humanized mouse model, Scientific Reports 9(1): 17391.
- Fontaine M.A.C., Westra M.M., Bot I., Jin H., Franssen A.J.P.M., Bot M., Jager S.C.A. de, Dzhagalov I., He Y.W., Vlijmen B.J.M. van, Gijbels M.J.J., Reutelingsperger C.P., Berkel T.J.C. van, Sluimer J.C., Temmerman L. & Biessen E.A.L. (2019), Low human and murine Mcl-1 expression leads to a pro-apoptotic plaque phenotype enriched in giant-cells, Scientific Reports 9(1): 14547.
- Heijden T. van der, Bot I. & Kuiper J. (2019), The IL-12 cytokine family in cardiovascular diseases, Cytokine 122: 154188.
- Kritikou E., Heijden T. van der, Swart M., Duijn J. van, Slütter B., Wezel A., Smeets H.J., Maffia P., Kuiper J. & Bot I. (2019), Hypercholesterolemia induces a mast cell-CD4+ T cell interaction in atherosclerosis, Journal of Immunology 202(5): 1531-1539.
- Puijvelde G.H.M. van, Foks A.C., Bochove R.E. van, Bot I., Habets K.L.L., Jager S.C. de, Borg M.N.D. ter, Osch P. van, Boon L., Vos M., Waard V. de & Kuiper J. (2018), CD1d deficiency inhibits the development of abdominal aortic aneurysms in LDL receptor deficient mice, 13(1): e0190962.
- Amersfoort J., Schaftenaar F.H., Douna H., Santbrink P.J. van, Kröner M.J., Puijvelde G.H.M. van, Quax P.H.A., Kuiper J. & Bot I. (2018), Lipocalin-2 contributes to experimental atherosclerosis in a stage-dependent manner, Atherosclerosis 275: 214-224.
- Indhirajanti S., Daele P.L.A. van, Bos S., Mulder M.T., Bot I. & Lennep J.E.R. van (2018), Systemic mastocytosis associates with cardiovascular events despite lower plasma lipid levels, Atherosclerosis 268: 152-156.
- Duijn J. van, Kritikou E., Benne N., Heijden T. van der, Puijvelde G.H. van, Kröner M.J., Schaftenaar F.H., Foks A.C., Wezel A., Smeets H., Yagita H., Bot I., Jiskoot W., Kuiper J. & Slütter B. (2018), CD8+ T-cells contribute to lesion stabilization in advanced atherosclerosis by limiting macrophage content and CD4+ T-cell responses, Cardiovascular Research 115(4): 729-738.
- Amersfoort J., Douna H., Schaftenaar F.H., Foks A.C., Kröner M.J., Santbrink P.J. van, Puijvelde G.H.M. van, Bot I. & Kuiper J. (2018), Defective Autophagy in T Cells Impairs the Development of Diet-Induced Hepatic Steatosis and Atherosclerosis, Frontiers in Immunology 9: 2937.
- Kritikou E., Duijn J. van, Nahon J.E., Heijden T. van der, Bouwman M., Groeneveldt C., Schaftenaar F.H., Kröner M.J., Kuiper J., Puijvelde G.H.M. van & Bot I. (2018), Disruption of a CD1d-mediated interaction between mast cells and NKT cells aggravates atherosclerosis, Atherosclerosis 280: 132-139.
- Slütter B.A., Depuydt M.A.C., Duijn J. van, Bot I., Wezel A., Koppejan H., Toes R. & Kuiper J. (2018), Masscytometry identifies CD8 T-cell diversity in human atherosclerotic lesions, Atherosclerosis 275: e8.
- Ingen E. van, Foks A.C., Kröner M.J., Agrawal S., Kuiper J., Quax P.H.A., Bot I. & Nossent A.Y. (2018), Inhibition of microRNA-494 halts atherosclerotic plaque progression and stabilizes advanced atherosclerotic lesions, Cardiovascular Research 114(suppl_1): S57-S57.
- Schaftenaar F.H., Amersfoort J., Douna H., Kroner M.J., Slutter B.A., Bot I., Puijvelde G.H.M. van & Kuiper J. (2018), Vaccination with APOB100 derived HLA-A2 restricted CD8 T cell epitopes did not reduce atherosclerosis in male LDLRKO HAPOB100TG HLA-A2TG mice, Atherosclerosis Supplements 32: 100-101.
- Amersfoort J., Kuiper J. & Bot I. (2018), Reply to "Lipocalin-2 contributes to experimental atherosclerosis in a stage-dependent manner", Atherosclerosis 278: 323-324.
- Tsiantoulas D., Bot I., Ozsvar-Kozma M., Goederle L., Perkmann T., Hartvigsen K., Conrad D.H., Kuiper J., Mallat Z. & Binder C.J. (2017), Increased Plasma IgE Accelerate Atherosclerosis in Secreted IgM Deficiency, Circulation Research 120(1): 78-84.
- Bot I. & Kuiper J. (2017), Stressed brain, stressed heart?, The Lancet 389(10071): 770-771.
- Bot I., Ortiz Zacarias N.V., Witte W.E. de, Vries H. de, Santbrink P.J. van, Velden D. van der, Kroener M.J., Berg D.J. van den, Stamos D., Lange E.C.M. de, Kuiper J., IJzerman A.P. & Heitman L.H. (2017), A novel CCR2 antagonist inhibits atherogenesis in apoE deficient mice by achieving high receptor occupancy, Scientific Reports 2017(7): 52.
- Kritikou E., Heijden T. van der, Swart M., Duijn J. van, Slutter B., Smeets H., Maffia P., Kuiper J. & Bot I. (2017), Hypercholesterolemia induces a mast cell-CD4+ T-cell interaction in atherosclerosis, Atherosclerosis 263: E124-E124.
- Heijden T. van der, Kritikou E., Venema W., Duijn J. van, Santbrink P.J. van, Slütter B., Foks A.C., Bot I. & Kuiper J. (2017), NLRP3 Inflammasome Inhibition by MCC950 Reduces Atherosclerotic Lesion Development in Apolipoprotein E-Deficient Mice-Brief Report, Arteriosclerosis, Thrombosis, and Vascular Biology 37(8): 1457-1461.
- Foks A.C. & Bot I. (2017), Preface: Pathology and Pharmacology of Atherosclerosis, European Journal of Pharmacology 816: 1-2.
- Welten S.M.J., Jong R.C.M. de, Wezel A., Vries M.R. de, Boonstra M.C., Parma L., Jukema J.W., Sluis T.C. van der, Arens R., Bot I., Agrawal S., Quax P.H.A. & Nossent A.Y. (2017), Inhibition of 14q32 microRNA miR-495 reduces lesion formation, intimal hyperplasia and plasma cholesterol levels in experimental restenosis, Atherosclerosis 261: 26-36.
- Temmerman L., Westra M.M., Bot I., Vlijmen B.J.M. van, Bree N. van, Bot M., Habets K.L.L., Keulers T.G.H., Vlag J. van der, Cotter T.G., Berkel T.J.C. van & Biessen E.A.L. (2017), Leukocyte Bim deficiency does not impact atherogenesis in ldlr(-/-) mice, despite a pronounced induction of autoimmune inflammation, Scientific Reports 7: 3086.
- Kovanen P.T. & Bot I. (2017), Mast cells in atherosclerotic cardiovascular disease - Activators and actions, European Journal of Pharmacology 816: 37-46.
- Shi G.P., Bot I. & Kovanen P.T. (2017), The complexity of substance P-mediated mast cell activation Reply, Nature Reviews Cardiology 14(2): 124.
- Aarup A., Nielsen C.H., Bisgaard L.S., Bot I., El-Ali H.H., Kjaer A., Nielsen L.B. & Pedersen T.X. (2017), Uremia does not affect neointima formation in mice, Scientific Reports 7: 6496.
- Bruin R.G. de, Shiue L., Prins J., Boer H.C. de, Singh A., Fagg W.S., Gils J.M. van, Duijs J.M., Katzman S., Kraaijeveld A.O., Böhringer S., Leung W.Y., Kielbasa S.M., Donahue J.P., Zande P.H. van der, Sijbom R., Alem C.M. van, Bot I., Kooten C. van, Jukema J.W., Esch H. van, Rabelink T.J., Kazan H., Biessen E.A., Ares M., Zonneveld A.J. van & Veer E.P. van der (2016), Quaking promotes monocyte differentiation into pro-atherogenic macrophages by controlling pre-mRNA splicing and gene expression, Nature Communications 2016(7): 10846.
- Foks A.C., Puijvelde G.H.M. van, Wolbert J., Kröner M.J., Frodermann V., Heijden T. van der, Santbrink P.J. van, Boon L., Bot I. & Kuiper J. (2016), CD11b+Gr-1+ myeloid-derived suppressor cells reduce atherosclerotic lesion development in LDLr deficient mice, Cardiovascular Research 111(3): 252-261.
- Frodermann V., Duijn J. van, Puijvelde G.H.M. van, Santbrink P.J. van, Lagraauw H.M., Vries M.R. de, Quax P.H.A., Bot I., Foks A.C., Jager S.C. de & Kuiper J. (2016), Heat-killed Staphylococcus aureus reduces atherosclerosis by inducing anti-inflammatory macrophages, Journal of Internal Medicine 279(6): 592-605.
- Lagraauw H.M., Wezel A., Velden D. van der, Kuiper J. & Bot I. (2016), Stress-induced mast cell activation contributes to atherosclerotic plaque destabilization, Journal of Immunology 196(1 supplement): 197.4.
- Suurmond J., Velden D. van der, Kuiper J., Bot I. & Toes R.E. (2016), Mast cells in rheumatic disease, European Journal of Pharmacology 778: 116-124.
- Kritikou E., Kuiper J., Kovanen P.T. & Bot I. (2016), The impact of mast cells on cardiovascular diseases, European Journal of Pharmacology 778: 103-115.
- Bot I. & Kuiper J. (2016), Relaxing the artery: A new strategy to limit atherogenesis, Atherosclerosis 251: 510-511.
- Bot I. & Kuiper J. (2016), The origin of atherosclerotic plaque cells: Plasticity or not?, Atherosclerosis 251: 536-537.
- Velden D. van der, Lagraauw H.M., Wezel A., Launay P., Kuiper J., Huizinga T.W.J., Toes R.E.M., Bot I. & Stoop J.N. (2016), Mast cell depletion in the preclinical phase of collagen-induced arthritis reduces clinical outcome by lowering the inflammatory cytokine profile, Arthritis Research & Therapy 18(1): 138.
- Tsiantoulas D., Bot I., Kiss M., Bartolini-Gritti B., Goederle L., Ozsvar-Kozma M., Perkmann T., Hartvigsen K., Bergthaler A., Conrad D.H., Kuiper J., Mallat Z., Jumaa H. & Binder C.J. (2016), Deficiency of secreted IgM antibodies causes increased plasma IgE levels that promote atherosclerosis, Atherosclerosis 252: E262-E263.
- Wezel A., Vries M.R. de, Maassen J.M., Kip P., Peters E.A., Karper J.C., Kuiper J., Bot I. & Quax P.H.A. (2016), Deficiency of the TLR4 analogue RP105 aggravates vein graft disease by inducing a pro-inflammatory response, Scientific Reports 6: 24248.
- Kritikou E., Puijvelde G.H.M. van, Heijden T. van der, Santbrink P.J. van, Swart M., Schaftenaar F.H., Kroner M.J., Kuiper J. & Bot I. (2016), Inhibition of lysophosphatidic acid receptors 1 and 3 attenuates atherosclerosis development in LDL-receptor deficient mice, Scientific Reports 6: 37585.
- Bot I., Shi G.-P. & Kovanen P.T. (2015), Mast Cells as Effectors in Atherosclerosis, Arteriosclerosis, Thrombosis, and Vascular Biology 35(2): 265-271.
- Frodermann V., Duijn J. van, Pel M. van, Santbrink P.J. van, Bot I., Kuiper J. & Jager S.C. de (2015), Mesenchymal Stem Cells Reduce Murine Atherosclerosis Development, Scientific Reports 5: 15559.
- Wezel A., Lagraauw H.M., Velden D. van der, Jager S.C. de, Quax P.H.A., Kuiper J. & Bot I. (2015), Mast cells mediate neutrophil recruitment during atherosclerotic plaque progression, Atherosclerosis 241(2): 289-296.
- Wezel A., Welten S.M., Razawy W., Lagraauw H.M., Vries M.R. de, Goossens E.A., Boonstra M.C., Hamming J.F., Kandimalla E.R, Kuiper J., Quax P.H.A, Nossent A.Y. & Bot I. (2015), Inhibition of MicroRNA-494 Reduces Carotid Artery Atherosclerotic Lesion Development and Increases Plaque Stability, Annals of Surgery 262(5): 841-848.
- Medina I., Cougoule C., Drechsler M., Bermudez B., Koenen R.R., Sluimer J., Wolfs I., Döring Y., Herias V., Gijbels M., Bot I., De Jager S.C., Weber C., Cleutjens J., Van Berkel T.J.C., Sikkink K.J., Mócsai A., Maridonneau-Parini I., Soehnlein O. & Biessen E.A. (2015), Hck/Fgr Kinase Deficiency Reduces Plaque Growth and Stability by Blunting Monocyte Recruitment and Intraplaque Motility, Circulation 132(6): 490-501.
- Lagraauw H.M., Kuiper J. & Bot I. (2015), Acute and chronic psychological stress as risk factors for cardiovascular disease: Insights gained from epidemiological, clinical and experimental studies, Brain, Behavior, and Immunity 50: 18-30.
- Frodermann V., Puijvelde G.H. van, Wierts L., Lagraauw H.M., Foks A.C., Santbrink P.J. van, Bot I., Kuiper J. & Jager S.C. de (2015), Oxidized low-density lipoprotein-induced apoptotic dendritic cells as a novel therapy for atherosclerosis, Journal of Immunology 194(5): 2208-2218.
- Shi G.P., Bot I. & Kovanen P.T. (2015), Mast cells in human and experimental cardiometabolic diseases, Nature Reviews Cardiology 12: 643-658.
- Wezel A., Quax P.H., Kuiper J. & Bot I. (2015), The role of mast cells in atherosclerosis, Hämostaseologie 35(2): 113-120.
- Ackers-Johnson M., Talasila A., Sage A.P., Long X., Bot I., Morrell N.W., Bennett M.R., Miano J.M. & Sinha S. (2015), Myocardin regulates vascular smooth muscle cell inflammatory activation and disease, Arteriosclerosis, Thrombosis, and Vascular Biology 35(4): 817-828.
- Wezel A., Velden D. van der, Maassen J.M., Lagraauw H.M., Vries M.R. de, Karper Ja., Kuiper J., Bot I. & Quax P.H.A. (2015), RP105 deficiency attenuates early atherosclerosis via decreased monocyte influx in a CCR2 dependent manner, Atherosclerosis 238(1): 132-139.
- Maiwald S., Sivapalaratnam S., Motazacker M.M., Capelleveen J.C. van, Bot I., Jager S.C. de, Eck M. van, Jolley J., Kuiper J., Stephens J., Albers C.A., Vosmeer C.R., Kruize H., Geerke D.P., Wal A.C. van der, Loos C.M. van der, Kastelein J.J., Trip M.D., Ouwehand W.H., Dallinga-Thie G.M. & Hovingh G.K. (2014), Mutation in KERA identified by linkage analysis and targeted resequencing in a pedigree with premature atherosclerosis, PLoS ONE 9(5): e98289.
- Bot I., Daissormont I.T., Zernecke A., Puijvelde G.H.M. van, Kramp B., Jager S.C. de, Sluimer J.C., Manca M., Hérias V., Westra M.M., Bot M., Santbrink P.J. van, Berkel T.J.C. van, Su L., Skjelland M., Gullestad L., Kuiper J., Halvorsen B., Aukrust P., Koenen R.R., Weber C. & Biessen E.A. (2014), CXCR4 blockade induces atherosclerosis by affecting neutrophil function, Journal of Molecular and Cellular Cardiology 74: 44-52.
- Rensing K.L., De Jager S.C., Stroes E.S., Vos M., Twickler M.T., Dallinga-Thie G.M., Vries C.J. de, Kuiper J., Bot I. & Thüsen J.H. von der (2014), Akt2/LDLr double knockout mice display impaired glucose tolerance and develop more complex atherosclerotic plaques than LDLr knockout mice, Cardiovascular Research 101(2): 277-287.
- Willems S., Velden D. van der, Quax P.H., Borst G.J. de, Vries J.P. de, Moll F.L, Kuiper J., Toes R.E, Jager S.C. de, Kleijn D.P. de, Hoefer I.E., Pasterkamp G. & Bot I. (2014), Circulating immunoglobulins are not associated with intraplaque mast cell number and other vulnerable plaque characteristics in patients with carotid artery stenosis, PLoS ONE 9(2): e88984.
- Wezel A., Vries M.R. de, Lagraauw H.M., Foks A.C., Kuiper J., Quax P.H.A. & Bot I. (2014), Complement factor C5a induces atherosclerotic plaque disruptions, Journal of Cellular and Molecular Medicine 18(10): 2020-2030.
- Wang Y., Parlevliet E.T., Geerling J.J., Van der Tuin S.J.L., Zhang H., Bieghs V., Jawad A.H.M., Shiri-Sverdlov R., Bot I., De Jager S.C.A., Havekes L.M., Romijn J.A., Willems van Dijk K. & Rensen P.C.N. (2014), Exendin-4 decreases liver inflammation and atherosclerosis development simultaneously by reducing macrophage infiltration, British Journal of Pharmacology 171(3): 723-734.
- Lagraauw H.M., Westra M.M., Bot M., Wezel A., Santbrink P.J. van, Pasterkamp G., Biessen E.A.L., Kuiper J. & Bot I. (2014), Vascular neuropeptide Y contributes to atherosclerotic plaque progression and perivascular mast cell activation, Atherosclerosis 235(1): 196-203.
- Segers Filip M.E., Den Adel Brigit, Bot Ilze, Van der Graaf Linda M., Van der Veer Eric P., Gonzalez Walter, Raynal Isabelle, De Winther Menno, Wodzig Will K., Poelmann Robert E., Van Berkel Theo J.C., Van der Weerd Louise & Biessen Erik A.L. (2013), Scavenger Receptor-AI-Targeted Iron Oxide Nanoparticles for In Vivo MRI Detection of Atherosclerotic Lesions, Arteriosclerosis, Thrombosis, and Vascular Biology 33(8): 1812-1819.
- Talasila Amarnath, Yu Haixiang, Ackers-Johnson Matthew, Bot Martine, Van Berkel Theo, Bennett Martin R., Bot Ilze & Sinha Sanjay (2013), Myocardin Regulates Vascular Response to Injury Through miR-24/-29a and Platelet-Derived Growth Factor Receptor-beta, Arteriosclerosis, Thrombosis, and Vascular Biology 33(10): 2355-2365.
- De Vries Margreet R., Wezel Anouk, Schepers Abbey, Van Santbrink Peter J., Woodruff Trent M., Niessen Hans W.M., Hamming Jaap F., Kuiper Johan, Bot Ilze & Quax Paul H.A. (2013), Complement factor C5a as mast cell activator mediates vascular remodelling in vein graft disease, Cardiovascular Research 97(2): 311-320.
- Karper J.C., De Jager S.C.A., Ewing M.M., De Vries M.R., Bot I., Van Santbrink P.J., Redeker A., Mallat Z., Binder C.J., Arens R., Jukema J.W., Kuiper J. & Quax P.H.A. (2013), An Unexpected Intriguing Effect of Toll-Like Receptor Regulator RP105 (CD180) on Atherosclerosis Formation With Alterations on B-Cell Activation, Arteriosclerosis, Thrombosis, and Vascular Biology 33(12): 2810-2817.
- Foks A.C., Ran I.A., Wasserman L., Frodermann V., Borg M.N. ter, Jager S.C. de, Santbrink P.J. van, Yagita H., Akiba H., Bot I., Kuiper J. & Puijvelde G.H.M. van (2013), T-cell immunoglobulin and mucin domain 3 acts as a negative regulator of atherosclerosis, Arteriosclerosis, Thrombosis, and Vascular Biology 33(11): 2558-2565.
- Yu Haixiang, Segers Filip, Sliedregt-Bol Karen, Bot Ilze, Woltman Andrea M., Boross Peter, Verbeek Sjef, Overkleeft Herman, Van der Marel Gijs A., Van Kooten Cees, Van Berkel Theo J.C. & Biessen Erik A.L. (2013), Identification of a novel CD40 ligand for targeted imaging of inflammatory plaques by phage display, The FASEB Journal 27(10): 4136-4146.
- Foks A.C., Puijvelde G.H.M. van, Bot I., Borg M.N. ter, Habets K.L., Johnson J.L., Yagita H., Berkel T.J. van & Kuiper J. (2013), Interruption of the OX40-OX40 ligand pathway in LDL receptor-deficient mice causes regression of atherosclerosis, Journal of Immunology 191(9): 4573-4580.
- Bot M., Jager S.C., MacAleese L., Lagraauw H.M., Berkel T.J.C. van, Quax P.H.A., Kuiper J., Heeren R.M., Biessen E.A. & Bot I. (2013), Lysophosphatidic acid triggers mast cell-driven atherosclerotic plaque destabilization by increasing vascular inflammation, Journal of Lipid Research 54(5): 1265-1274.
- Otten Jeroen J.T., De Jager Saskia C.A., Kavelaars Annemieke, Seijkens Tom, Bot Ilze, Wijnands Erwin, Beckers Linda, Westra Marijke M., Bot Martine, Busch Matthias, Bermudez Beatriz, Van Berkel Theo J.C., Heijnen Cobi J. & Biessen Erik A.L. (2013), Hematopoietic G-protein-coupled receptor kinase 2 deficiency decreases atherosclerotic lesion formation in LDL receptor-knockout mice, The FASEB Journal 27(1): 265-276.
- Willems Sanne, Vink Aryan, Bot Ilze, Quax Paul H.A., De Borst Gert Jan, De Vries Jean-Paul P.M., Van de Weg Sander M., Moll Frans L., Kuiper Johan, Kovanen Petri T., De Kleijn Dominique P.V., Hoefer Imo E. & Pasterkamp Gerard (2013), Mast cells in human carotid atherosclerotic plaques are associated with intraplaque microvessel density and the occurrence of future cardiovascular events, European Heart Journal 34(48): 3699-3706.
- Van der Veer Eric P., De Bruin Ruben G., Kraaijeveld Adriaan O., De Vries Margreet R., Bot Ilze, Pera Tonio, Segers Filip M., Trompet Stella, Van Gils Janine M., Roeten Marko K., Beckers Cora M., Van Santbrink Peter J., Janssen Anique, Van Solingen Coen, Swildens Jim, De Boer Hetty C., Peters Erna A., Bijkerk Roel, Rousch Mat, Doop Merijn, Kuiper Johan, Schalij Martin Jan, Van der Wal Allard C., Richard Stephane, Van Berkel Theo J.C., Pickering J. Geoffrey, Hiemstra Pieter S., Goumans Marie Jose, Rabelink Ton J., De Vries Antoine A.F., Quax Paul H.A., Jukema J. Wouter, Biessen Erik A.L. & Van Zonneveld Anton Jan (2013), Quaking, an RNA-Binding Protein, Is a Critical Regulator of Vascular Smooth Muscle Cell Phenotype, Circulation Research 113(9): 1065-1075.
- Foks A.C., Ran I.A., Frodermann V., Bot I., Santbrink P.J. van, Kuiper J. & Puijvelde G.H.M. van (2013), Agonistic anti-TIGIT treatment inhibits T cell responses in LDLr deficient mice without affecting atherosclerotic lesion development, PLoS ONE 8(12): e83134.
- Bot M., Veldhoven P.P. van, Jager S.C.A. de., Johnson J., Nijstad N., Santbrink P.J. van, Westra M.M., Hoeven G. van der, Gijbels M.J., Mueller-Tidow C., Varge G., Tietge U.J.F., Kuiper J., Berkel T.J.C. van, Nofer J.R., Bot I. & Biessen E.A.L. (2013), Hematopoietic Sphingosine 1-Phosphate Lyase Deficiency Decreases Atherosclerotic Lesion Development in LDL-Receptor Deficient Mice, PLoS ONE 8(5): e63360.
- Karper J., De Jager S.C.A., Ewing M.M., De Vries M.R., Arens R., Bot I., Jukema J.W., Kuiper J. & Quax P. (2013), RP105 (cd180) as a TLR-4 regulator ameliorates atherosclerosis via its role on B-cells, European Heart Journal 34: 441-441.
- Schuh Alexander, Kroh Andreas, Konschalla Simone, Liehn Elisa A., Sobota Radoslav M., Al Biessen Erik, Bot Ilze, Taha Soenmez Tolga, Schober Andreas, Marx Nikolaus, Weber Christian & Sasse Alexander (2012), Myocardial regeneration by transplantation of modified endothelial progenitor cells expressing SDF-1 in a rat model, Journal of Cellular and Molecular Medicine 16(10): 2311-2320.
- De Waard Vivian, Marinkovic Goran, Bot Ilze, De Jager Saskia C.A., Talib Sara, Egashira Kensuke, De Vries Margreet R., Quax Paul H.A., Biessen Erik A.L. & Van Berkel Theo J.C. (2012), Systemic MCP1/CCR2 blockade and leukocyte specific MCP1/CCR2 inhibition affect aortic aneurysm formation differently, Vascular Pharmacology 56(5-6): 380-380.
- Bot I., Vries H. de, Bot M., Berkel T.J.C. van, IJzerman A.P. & Kuiper J. (2012), Adenosine A(2B) receptor agonism inhibits vascular smooth muscle cell proliferation and intimal hyperplasia in ApoE deficient mice, Vascular Pharmacology 56(5-6): 349-350.
- Vries M.R. de, Wezel A., Schepers A., Kuiper J., Bot I. & Quax P.H.A. (2012), Mast cell activation via complement factor C5a modulates vein graft remodeling and accelerated atherosclerosis, Vascular Pharmacology 56(5-6): 372-372.
- Dekker W.K. den, Tempel D., Bot I., Biessen E.A., Joosten L.A., Netea M.G., Meer J.W.M. van der, Cheng C. & Duckers H.J. (2012), Mast Cells Induce Vascular Smooth Muscle Cell Apoptosis via a Toll-Like Receptor 4 Activation Pathway, Arteriosclerosis, Thrombosis, and Vascular Biology 32(8): 1960-1969.
- Foks A.C., Bot I., Frodermann V., Jager S.C. de, Borg M. ter, Santbrink P.J. van, Yagita H., Kuiper J. & Puijvelde G.H.M. van (2012), Interference of the CD30-CD30L pathway reduces atherosclerosis development, Arteriosclerosis, Thrombosis, and Vascular Biology 32(12): 2862-2868.
- Jager S.C. de, Karper J.C., Bot I., Vries M.R. de, Binder C.J., Kuiper J. & Quax P.H. (2012), Extracellular toll like receptor regulator RP105/CD180 ameliorates atherosclerosis via its role on B cells, Immunology 137: 296-296.
- Foks A.C., Bot I., Frodermann V., Jager S.C.A. de, Borg M. ter, Santbrink P.J. van, Yagita H., Kuiper J. & Puijvelde G.H.M. van (2012), Interference of the CD30-CD30L pathway reduces atherosclerosis development, Immunology 137: 478-478.
- Foks A.C., Frodermann V., Ter Borg M., Habets K.L., Bot I., Zhao Y., Van Eck M., Van Berkel T.J., Kuiper J. & Van Puijvelde G.H. (2011), Differential effects of regulatory T cells on the initiation and regression of atherosclerosis, Atherosclerosis 218(1): 53-60.
- Jager S.C. de, Bermudez B., Bot I., Koenen R.R., Bot M., Kavelaars A., Waard V. de, Heijnen C.J., Muriana F.J., Weber C., Berkel T.J.C. van, Kuiper J., Lee S.J., Abia R. & Biessen E.A. (2011), Growth differentiation factor 15 deficiency protects against atherosclerosis by attenuating CCR2-mediated macrophage chemotaxis, The Journal of Experimental Medicine 208(2): 217-225.
- Van Puijvelde G., Van Osch P., Bot I., Foks A., Ter Borg M., Habets K., Van Berkel T. & Kuiper J. (2011), NKT cells aggravate the development of abdominal aortic aneurysms, Immunology 135: 173-173.
- Wezel A., De Vries M.R., Schepers A., Kuiper J., Bot I. & Quax P. (2011), Complement-factor C5a modulates vein graft remodeling and accelerated atherosclerosis via interference in Mast cell activation, Molecular Immunology 48(14): 1693-1693.
- Bot I. & Biessen E.A. (2011), Mast cells in atherosclerosis, Thrombosis and Haemostasis 106(5): .
- Vries M.R. de, Wezel A., Schepers A., Kuiper J., Bot I. & Quax P.H.A. (2011), Complement factor C5A modulates vein graft remodeling and accelerated atherosclerosis via interference in mast cell activation, Atherosclerosis Supplements 12(1): 79-79.
- De Waard V., Bot I., De Jager S.C., Talib S., Egashira K., De Vries M.R., Quax P.H., Biessen E.A. & Van Berkel T.J. (2010), Systemic MCP1/CCR2 blockade and leukocyte specific MCP1/CCR2 inhibition affect aortic aneurysm formation differently, Atherosclerosis 211(1): .
- Bot M., Bot I., Lopez-Vales R., Van de Lest C.H., Saulnier-Blache J.S., Helms J.B., David S., Van Berkel T.J. & Biessen E.A. (2010), Atherosclerotic lesion progression changes lysophosphatidic acid homeostasis to favor its accumulation, The American Journal of Pathology 176(6): .
- Bot I., Bot M., Van Heiningen S.H., Van Santbrink P.J., Lankhuizen I.M., Hartman P., Gruener S., Hilpert H., Van Berkel T.J., Fingerle J. & Biessen E.A. (2010), Mast cell chymase inhibition reduces atherosclerotic plaque progression and improves plaque stability in ApoE-/- mice, Cardiovascular Research 89(1): .
- Bot I., Jukema J.W., Lankhuizen I.M., Van Berkel T.J. & Biessen E.A. (2010), Atorvastatin inhibits plaque development and adventitial neovascularization in ApoE deficient mice independent of plasma cholesterol levels, Atherosclerosis 214(2): .
- Bot I., De Jager S.C.A., Bot M., Van Heiningen S.H., Van Berkel T.J.C., Von der Thusen J.H. & Biessen E.A.L. (2010), SUBSTANCE P MEDIATED ADVENTITIAL MAST CELL ACTIVATION INDUCES INTRAPLAQUE HEMORRHAGE IN ADVANCED ATHEROSCLEROSIS, Atherosclerosis Supplements 11(2): 2-2.
- Es T. van, Puijvelde G.H.M. van, Foks A.C., Habets K.L., Bot I., Gilboa E., Berkel T.J. van & Kuiper J. (2010), Vaccination against Foxp3(+) regulatory T cells aggravates atherosclerosis, Atherosclerosis 209(1): 74-80.
- Borensztajn K., Bresser P., Loos C. van der, Bot I., Blink B. van den, Bakker M.A. den, Daalhuisen J., Groot A.P., Peppelenbosch M.P., Thusen J.H. von der & Spek C.A. (2010), Protease-Activated Receptor-2 Induces Myofibroblast Differentiation and Tissue Factor Up-Regulation during Bleomycin-Induced Lung Injury Potential Role in Pulmonary Fibrosis, The American Journal of Pathology 177(6): 2753-2764.
- Van Es T., Van Puijvelde G.H., Foks A.C., Habets K.L., Bot I., Gilboa E., Van Berkel T.J. & Kuiper J. (2010), Vaccination against Foxp3(+) regulatory T cells aggravates atherosclerosis, Atherosclerosis 209(1): .
- Jager S.C. de, Canté-Barrett K., Bot I., Husberg C., Puijvelde G.H.M. van, Santbrink P.J. van, Yndestad A., Oever J.M. van den, Kuiper J., Berkel T.J.C. van, Lipp M., Zwaginga J.J., Fibbe W.E., Aukrust P. & Biessen E.A. (2009), Impaired effector memory T-cell regulation facilitates graft versus host disease in CCR7-deficient bone marrow transplant chimeras, Transplantation 88(5): 631-639.
- Guo J., Bot I., De Nooijer R., Hoffman S., Stroup G.B., Biessen E.A.L., Benson G.M., Groot P. de, Van Eck M. & Van Berkel T.J.C. (2009), Leucocyte cathepsin K affects atherosclerotic lesion composition and bone mineral density in low-density lipoprotein receptor deficient mice, Cardiovascular Research 81(2): 278-85.
- Lucas Alexandra, Liu Liying, Dai Erbin, Bot Ilze, Viswanathan Kasinath, Munuswamy-Ramunujam Ganesh, Davids Jennifer A., Bartee Mee Y., Richardson Jakob, Christov Alexander, Wang Hao, Macaulay Colin, Poznansky Mark, Zhong Robert, Miller Leslie, Biessen Erik, Richardson Mary, Sullivan Collin, Moyer Richard, Hatton Mark, Lomas David A. & McFadden Grant (2009), The Serpin Saga; Development of a New Class of Virus Derived Anti-Inflammatory Protein Immunotherapeutics, Advances in Experimental Medicine and Biology 666: 132-156.
- Eefting D., Bot I., De Vries M., Schepers A., Van Bockel J.H., Van Berkel T.J.C., Biessen E.A.L. & Quax P. (2009), Local lentiviral short hairpin RNA silencing of CCR2 inhibits vein graft thickening in hypercholesterolemic apolipoprotein E3-Leiden mice, Journal of Vascular Surgery 50(1): 152-160.
- De Nooijer R., Bot I., Von der Thusen J.H., Leeuwenburgh M.A., Overkleeft H., Kraaijeveld A.O., Dorland R., Van Santbrink P.J., Van Heiningen S.H., Westra M.M., Kovanen P.T., Jukema J.W., Van der Wall E.E., Van Berkel T.J.C., Shi G.P. & Biessen E.A.L. (2009), Leukocyte Cathepsin S Is a Potent Regulator of Both Cell and Matrix Turnover in Advanced Atherosclerosis, 29(2): 188-194.
- Kraaijeveld A.O., Jager S.C.A. de, Daissormont I., Bot I.M., Segers F., Mccoll S.R., Atamas S.P., Berkel T.J.C. van, Jukema J.W. & Biessen E.A.L. (2008), CC chemokine ligand-18 enhances atherogenesis and induces accumulation of T-cells in atherosclerotic plaques of ApoE-/- mice, European Heart Journal 29(Suppl_1): 524-524.
- Zernecke A., Bot I., Djalali-Talab Y., Shagdarsuren E., Bidzhekov K., Meiler S., Krohn R., Schober A., Sperandio M., Soehnlein O., Bornemann J., Tacke F., Biessen E.A.L. & Weber C. (2008), Protective role of CXC receptor 4/CXC ligand 12 unveils the importance of neutrophils in atherosclerosis, Circulation Research 102(2): 209-217.
- Bot I., Berkel T.J.C. van & Biessen E.A.L. (2007), Viral serine protease inhibitors as anti-atherosclerotic therapy, Current Opinion in Investigational Drugs 8: 729-735.
- Krohn Regina, Raffetseder Ute, Bot Ilze, Zerneeke Alma, Shagdarsuren Erdenechimeg, Liehn Elisa A., Van Santbrink Peter J., Nelson Peter J., Biessen Erik A., Mertens Peter R. & Weber Christian (2007), Y-box binding protein-1 controls CC chemokine ligand-5 (CCL5) expression in smooth muscle cells and contributes to neointima formation in atherosclerosis-prone mice, Circulation 116(16): 1812-1820.
- Raffetseder Ute, Krohn Regina, Bot Ilze, Biesse Erik, Weber Christian & Mertens Peter R. (2007), YB-1 controls RANTES expression in smooth muscle cells contributing to neointima formation in atheroslerosis-prone mice, Nephrology Dialysis Transplantation 22: 220-221.
- Lucerna M., Zernecke A., Nooijer R. de, Jager S.C.A. de, Bot I., Lans C. van der, Kholova I., Liehn E.A., Berkel T.J.C. van, Yla-Herttuala S., Weber C. & Biessen E.A.L. (2007), Vascular endothelial growth factor-A induces plaque expansion in Apo Eknockout mice by promoting de novo leukocyte recruitment, Blood 109: 122-129.
- Bot I., Jager S.C. de, Zernecke A., Weber C., Berkel T.J.C. van & Biessen E.A. (2007), Mast cell activation promotes leukocyte recruitment and adhesion to the atherosclerotic plaque, Circulation 116(16): 242-242.
- Jager S.C. de, Bermudez B., Bot I., Muriana F.J., Berkel T.J. van, Lee S.J., Abia R. & Biessen E.A. (2007), Hematopoietic deficiency of macrophage inhibitory cytokine-1 affects CCR2 function and attenuates plaque leukocyte accumulation in LDL receptor knockout mice, Circulation 116(16): 171-171.
- Yu Haixiang, Bot Ilze, Sliedregt Karen, Xu Xingfu, Van der Marel Gijs A., Overkleeft Herman S., Van Berkel Theo J. & Biessen Erik A. (2007), Rational design of a potent bipartite inhibitor of nuclear factor of activated T cells (NFAT), a key regulator of cardiovascular hyperplasia, Circulation 116(16): 246-246.
- Bot I., Jager S.C.A. de, Zernecke A., Lindstedt K., Berkel T.J.C. van, Weber C. & Biessen E.A.L. (2007), Perivascular mast cells promote atherogenesis and induce plaque destabilization in apolipoprotein E-deficient mice, Circulation 115: 2516-2525.
- De Nooijer Ramon, Lucerna Marcus, De Jager Saskia, Bot Ilze, Kraaijeveld Adriaan, Lutters Bianca, Van Santbrink Peter, Jukema Wouter J., Van Berkel Theo J. & Biessen Erik A. (2006), Murine tissue factor pathway inhibitor-2 aggravates injury-induced hyperplasia but reduces plaque stability, Circulation 114(18): 70-70.
- Bot M., Bot I., Jager S.C. de, Berkel T.J.C. van & Biessen E.A. (2006), Lysophosphatidic acid: a two-faced key actor in atherogenesis, Circulation 114(18): 341-341.
- Bot I., Zernecke A., Berkel T.J. van, Weber C. & Biessen E.A. (2006), Lentiviral blockade of the SDF-1 alpha/CXCR4 pathway in leukocytes aggravates atherosclerotic lesion development, Circulation 114(18): 110-110.
- Bot l., Eefting D., Vries M.R. de, Bockel H. van, Berkel T.J.C. van, Biessen E.A. & Quax P.H. (2006), Local lentiviral ShRNA mediated silencing of CCR2 inhibits intimal hyperplasia in ApoE3Leiden mice, Circulation 114(18): 122-123.
- Eefting D., Bot I., Vries M.R. de, Bockel J.H. van, Berkel T.J.C. van, Biessen E.A.L. & Quax P.H.A. (2006), Local lentiviral shRNA silencing of CCR2 inhibits vein graft thickening in hypercholesterolemic ApoE3Leiden mice, European Heart Journal 27(suppl_1): 5.
- Bot M., Bot I., Berkel T.J.C. van & Biessen E.A.L. (2006), Atherosclerotic lesion progression changes lysophosphatidic acid homeostasis to favor its accumulation, Atherosclerosis Supplements 7(3): 230-230.
- Bot I., De Jager S.C.A., Van Berkel T.J.C. & Biessen E.A.L. (2006), Systemic mast cell activation induces atherosclerotic plaque progression in ApoE-/- mice, Atherosclerosis Supplements 7(3): 247-247.
- De Boer H.C., Verseyden C., Ulfman L.H., Zwaginga J.J., Bot I., Biessen E.A., Rabelink T.J. & Van Zonneveld A.J. (2006), Fibrin and activated platelets cooperatively guide stem cells to a vascular injury and promote differentiation towards an endothelial cell phenotype, Arteriosclerosis, Thrombosis, and Vascular Biology 26(7): 1653-1659.
- Bot I. (22 September 2005), Modulation of Atherothrombotic Factors: Novel Strategies for Plaque Stabilization (Dissertatie. Leiden Centre for Drug Research, Faculty of Science, Leiden University). Supervisor(s) and Co-supervisor(s): Berkel T.J.C. van, Biessen E.A.L.
- Donners M.M., Bot I., Windt L.J. de, Berkel T.J.C. van, Daemen M.J., Biessen E.A.L. & Heeneman S. (2005), Low-dose FK506 blocks collar-induced atherosclerotic plaque development and stabilizes plaques in ApoE-/- mice, American Journal of Transplantation 5: 1204-1215.
- Zernecke A., Schober A., Bot I., Hundelshausen P. von der, Liehn E.A., Moepps B., Mericskay M., Gierschik P., Biessen E.A.L. & Weber C. (2005), SDF-1alpha/CXCR4 axis is instrumental in neointimal hyperplasia and recruitment of smooth muscle progenitor cells, Circulation Research 96: 784-791.
- Bot I., Guo J., Eck M. van, Santbrink P.J. van, Groot P.H., Hildebrand RB, Seppen J., Berkel T.J.C. van & Biessen E.A.L. (2005), Lentiviral shRNA silencing of murine bone marrow cell CCR2 leads to persistent knockdown of CCR2 function in vivo, Blood 106: 1147-1153.
- Zernecke A., Schober A., Bot I., Liehn E.A., Hundelshausen P. von der, Biessen E.A. & Weber C. (2005), Instrumental role of the stromal cell derived factor-1/CXCR4 axis in neointimal recruitment of smooth muscle cell progenitors after arterial injury, European Heart Journal 26(Suppl_1): 237-237.
- Lucerna M., Nooijer R. de, Zernecke A., Bot I., Jager S. de, Berkel T. van, Weber C. & Biessen E. (2005), Adenoviral mediated overexpression of VEGF leads to a vulnerable plaque phenotype in ApoE-deficient mice, Journal of Vascular Research 42(Supplement 2): 111-112.
- Donners M., Heeneman S., Bot I., Hooff J. van, Windt L. de, Berkel T.J.C. van, Daemen M. & Biessen E. (2004), FK506 immunosuppression inhibits collar-induced atherosclerotic plaque development in ApoE deficient mice, American Journal of Transplantation 4(s8): 192-193.
- Florea B.I., Molenaar T.J.M., Bot I., Michon I.N., Kuiper J., Berkel T.J.C. van, Junginger H.E., Biessen E.A.L. & Borchard G. (2003), Identification of an internalising peptide in differentiated Calu-3 cells by phage display technology; application to gene delivery to the airways, Journal of Drug Targeting 11(7): 383-390.
- Zelcer N., Saeki T., Bot I., Kuil A. & Borst P. (2003), Transport of bile acids in multidrug-resistance-protein 3-overexpressing cells co-transfected with the ileal Na+-dependent bile-acid transporter, Biochemical Journal 369: 23-30.
- Bot I., Thüsen J.H. von der, Donners M.M., Lucas A., Fekkes M.L., Jager S.C.A. de, Kuiper J., Daemen M.J., Berkel T.J.C. van, Heeneman S. & Biessen E.A.L. (2003), Serine protease inhibitor Serp-1 strongly impairs atherosclerotic lesion formation and induces a stable plaque phenotype in ApoE-/-mice, Circulation Research 93: 464-471.
- Bot I., Thüsen J.H. von der, Donners M.M., Lucas A.R., Berkel T.J.C. van, Kuiper J., Heeneman S. & Biessen E.A.L. (2002), The serine protease inhibitor Serp-1 strongly impairs de novo atherosclerotic lesion formation in ApoE -/- mice, Circulation 106: II-300.
- Editorial Board Member Vascular Pharmacology
- Editorial Board Member Arteriosclerosis, Thrombosis, and Vascular Biology
- Senior Editorial Board Member