Metabolomics as a tool to investigate natural products

10:00 – 11:00 Introduction to metabolomics (Robert Verpoorte) 
11:15 – 12:45  Application of metabolomics to medicinal plant research (Alvaro Viljoen)
14:00 – 14:45 Revisiting traditional chinese medicine by metabolomics (Mei Wang)

How to prepare samples, extract, separate metabolites, and how to process analytical data for statistical and multivariate data analysis

10:00 – 11:00 Sample preparation (Hye Kyong Kim)

11:00 – 12:00 Sample preparation and extraction (Ria: NPL lab; Kim: 1.5.03)

• Divide into two groups (A&B)
  •  11:00-11:30: Group A: watch video: sample preparation with Kim (1.5.03). Group B: Demonstration in the lab with Ria (NPL lab).
  • 11.30-12:00: switch

12:00 - 12:30 Q & A for sample preparation and extraction (Kim& Ria, 1.5.03, if necessary)

How to separate metabolites

10:00 – 11:15 Basic chromatography theory for liquid chromatography (Erica G. Wilson)

14:00 - 16:00 Basic of multivariate data analysis (Harald van Mil)

How to process analytical data for statistical and multivariate data analysis

10:00 – 12:00 TLC application to medicinal plant metabolic fingerprinting (Tien Do)

12:15 – 13:00 Chromatography and spectroscopy data processing for metabolomics (Jeroen Jansen)

Practice of NMR and NMR meassurements

10:00 – 11:30 Identification of Metabolites by MS spectrometry (Rob van der Heijden)

13:00 – 14:00 LC-MS demonstration (Ozlem, 4th floor LC room)

10:00 – 12:00 Gas Chromatography-based metabolomics: theory and applications (Joachim Kopka)

14:00 – 15:00 GC-MS and LC-MS data processing (Hocelayne Paulino Fernandes, and Özlem Erol, room 1.5.03)

15:15 – 15:45 Q & A for LC-MS data processing (if necessary)

How to analyse metabolites by NMR

10:00 – 12:00 NMR based metabolomics: Theory and Applications (Jan Schripsema)

14:00 – 15:00 NMR facility demonstration (Young Hae Choi)

How to apply metabolomics to plant sciences

10:00 – 12:00 Integrating metabolomic data from multiple analytical platforms for a comprehensive characterization of lemon essential oil (Serge Rudaz)

14:00 – 16:00 Strategies for unambiguous identification of biomarkers

Structure elucidation of amino acids I and II

10:00 – 12:00 Structural elucidation of amino acids I (Young Hae Choi)

14:00 – 16:00 Structural elucidation of amino acids II (Young Hae Choi)

Structure elucidation of amino acids III

10:00 – 12:00 Structural elucidation of amino acids III (Young Hae Choi)

Structure elucidation of sugars I and II

10:00 – 12:00 Structural elucidation of sugars (Young Hae Choi)

14:00 – 16:00 Structural elucidation of sugars (Young Hae Choi)

Additional MS-based techniques for metabolomics

10:00 – 12:00 DART mass as a tool of metabolic profiling technique (Young Pyo Jang)

14:00 – 16:00 Ambient mass spectrometry a useful tool for metabolomics (Teris van Beek)

How to apply metabolomics to clinical studies

10:00 – 11:30 Metabolomics in human health and disease (Martin Giera)

14:00 – 16:00 NMR-based profiling of intra- and extracellular metabolome (Sarantos Kostidis)

How to apply metabolomics to medicinal plants studies and concluding remarks

10:00 – 11:00 Metaboloimcs in Industry (Doris Jacobs)

11:15 – 12:00 Concluding remarks (Robert Verpoorte and Young Hae Choi)

12:15 – 13:00 Course evaluation