Gregg Siegal
Postdoc / guest
- Name
- Dr. G.D. Siegal
- Telephone
- +31 71 527 2727
- g.siegal@chem.leidenuniv.nl
Postdoc / guest
- Science
- Leiden Institute of Chemistry
- LIC/Chemical Biology
- Chaikuad A., Lang S., Brennan P.E., Temperini C., Fedorov O., Hollander J., Nachane R., Abell C., Muller S. & Siegal G.D. (2016), Structure-Based Identification of Inhibitory Fragments Targeting the p300/CBP-Associated Factor Bromodomain, Journal of Medicinal Chemistry 59(4): 1648-1653.
- Hameetman L., Fits L. van der, Zoutman W.H., Out-Luiting J.J., Siegal G.D., Esch I.J.P. de, Vermeer M.H. & Tensen C.P. (2015), EPHA4 is overexpressed but not functionally active in Sézary syndrome, OncoTarget 6(31): 31868-31876.
- Barelier S., Eidam O., Fish I., Hollander J.G., Figaroa F.A., Nachane R., Irwin J.J., Shoichet B.K. & Siegal G.D. (2014), Increasing Chemical Space Coverage by Combining Empirical and Computational Fragment Screens, ACS Chemical Biology 9(7): 1528-1535.
- Chen D., Ranganathan A., IJzerman A.P., Siegal G. & Carlsson J. (2013), Complementarity between in Silico and Biophysical Screening Approaches in Fragment-Based Lead Discovery against the A(2A) Adenosine Receptor, Journal of Chemical Information and Modeling 53(10): 2701-2714.
- Guan J.Y., Keizers P.H.J., Liu W.M., Loehr F., Skinner S.P., Heeneman E.A., Schwalbe H., Ubbink M. & Siegal G. (2013), Small-molecule binding sites on proteins established by paramagnetic NMR spectroscopy, Journal of the American Chemical Society 135(15): 5859-5868.
- Chen D., Errey J.C., Heitman L.H., Marshall F.H., IJzerman A.P. & Siegal G.D. (2012), Fragment screening of GPCRs using biophysical methods: identification of ligands of the adenosine A(2A) receptor with novel biological activity, ACS Chemical Biology 7(12): 2064-2073.
- Shah D.M., Ab E., Diercks T., Hass M.A.S., Nuland N.A.J. van & Siegal G. (2012), Rapid Protein-Ligand Costructures from Sparse NOE Data, Journal of Medicinal Chemistry 55(23): 10786-10790.
- Linden O.P.J. van, Farenc C., Zoutman W.H., Hameetman L., Wijtmans M., Leurs R., Tensen C.P., Siegal G. & Esch I.J.P. de (2012), Fragment based lead discovery of small molecule inhibitors for the EPHA4 receptor tyrosine kinase, European Journal of Medicinal Chemistry 47: 493-500.
- Farenc C. & Siegal G.D. (2012), Recent advances in biomolecular NMR for drug discovery. In: Clore M. & Potts J. (Eds.), Recent Developments in Biomolecular NMR. RSC Biomolecular Sciences. Cambridge, UK: Royal Society of Chemistry. 254-270.
- Farenc C., Celie P.H.N., Tensen C.P., Esch I.J.P. de & Siegal G.D. (2011), Crystal structure of the EphA4 protein tyrosine kinase domain in the apo- and dasatinib-bound state, FEBS Letters 585(22): 3593-3599.
- Congreve M., Rich R.L., Myszka D.G., Figaroa F., Siegal G. & Marshall F.H. (2011), Fragment screening of stabilized 6-protein-coupled receptors using biophysical methods, Methods in Enzymology 493: 115-136.
- Fruh V., IJzerman A.P. & Siegal G. (2011), How to Catch a Membrane Protein in Action: A Review of Functional Membrane Protein Immobilization Strategies and Their Applications, Chemical Reviews 111(2): 640-656.
- Groote F.H. de, Jansen J.G., Masuda Y., Shah D.M., Kamiya K., Wind N. de & Siegal G.D. (2011), The Rev1 translesion synthesis polymerase has multiple distinct DNA binding modes, DNA Repair 10(9): 915-925.
- Overmeer R.M., Gourdin A.M., Giglia-Mari A., Kool H., Houtsmuller A.B., Siegal G.D., Fousteri M.I., Mullenders L.H.F. & Vermeulen W. (2010), Replication Factor C Recruits DNA Polymerase delta to Sites of Nucleotide Excision Repair but Is Not Required for PCNA Recruitment, Molecular and Cellular Biology 30(20): 4828-4839.
- Fruh V., Zhou Y.P., Chen D., Loch C., Eiso A.B., Grinkova Y.N., Verheij H., Sligar S.G., Bushweller J.H. & Siegal G. (2010), Application of Fragment-Based Drug Discovery to Membrane Proteins: Identification of Ligands of the Integral Membrane Enzyme DsbB, Chemistry & Biology 17(8): 881-891.
- Kobayashi M., Eiso A.B., Bonvin Amjj & Siegal G.D. (2010), Structure of the DNA-bound BRCA1 C-terminal Region from Human Replication Factor C p140 and Model of the Protein-DNA Complex, Journal of Biological Chemistry 285(13): 10087-10097.
- Tuin A.W., Siegal G.D., Marel G.A. van der, Overkleeft H.S. & Filippov D.V. (2006), Facile synthesis and application of uniformly-13C, 15N - labeled phosphotyrosine for ligand binding studies, Bioorganic and Medicinal Chemistry Letters 16(14): 3806-3808.
- Kobayashi M., Figaroa F., Meeuwenoord N.J., Jansen L.E.T. & Siegal G.D. (2006), Characterization of the DNA binding and structural properties of the BRCT region of human replication factor C p140 subunit, Journal of Biological Chemistry 281(7): 4308-4317.
- Marquardsen T., Hofmann M., Hollander J.G., Loch C.M.P., Kiihne S.R., Engelke F. & Siegal G.D. (2006), Development of a dual cell, flow-injection sample holder, and NMR probe for comparative ligand-binding studies, Journal of Magnetic Resonance 182(1): 55-65.
- Vanwetswinkel S., Heetebrij R.J., Duynhoven J. van, Hollander J.G., Filippov D.V., Hajduk P.J. & Siegal G.D. (2005), TINS, Target Immobilized NMR Screening: an efficient and sensitive method for ligand discovery, Chemistry & Biology 12(2): 207-216.
- Hilge M., Siegal G.D., Vuister G.W., Guntert P., Gloor S.M. & Abrahams J.P. (2003), ATP-induced conformatinal changes of the nucleotide-binding domain of Na,K-ATPase, Nature Structural Biology 10: 468-474.
- Well R.M. van, Marinelli L., Altona C., Erkelens K., Siegal G.D., Raaij M.J. van, Llamas-Saiz A.L., Kessler H., Novellino E., Lavecchia A., Boom J.H. van & Overhand M. (2003), Conformational analysis of furanoid epsilon-sugar amino acid containing cyclic peptides by NMR spectroscopy, molecular dynamics simulation, and X-ray crystallography: Evidence for a novel turn structure, Journal of American Chemical Society 125(36): 10822-10829.
- Vanwetswinkel S., Kriek J., Andersen G.R., Guntert P., Dijk J., Canters G.W. & Siegal G.D. (2003), Solution structure of the 162 residue C-terminal domain of human elongation factor 1B gamma, Journal of Biological Chemistry 278(44): 43443-43451.
- Vanwetswinkel S., Kriek J., Andersen G.R., Dijk J. & Siegal G.D. (2003), 1H(15) and (13)C resonance assignments of the highly conserved 19kDa C-terminal domain from human Elongation Factor 1Bgamma, Journal of Biomolecular NMR 26: 189-190.
- Aguilera Diez M.B., Siegal G.D., Overkleeft H.S., Meeuwenoord N.J., Rutjes F.P.J.T., Hest J.C.M. van, Schoemaker H.E., Marel G.A. van der, Boom J.H. van & Overhand M. (2001), Synthesis and structural analysis of a b-hairpin modified peptide containing a sugar amino acid, European Journal of Organic Chemistry 2001(8): 1541-1574.
- Kristensen S., Siegal G.D., Davis B., Sankar A. & Driscoll P.C. (2000), Backbone dynamics of the C-terminal SH2 domain of the p85a subunit of phosphoinositide-3 kinase. Effect of phosphotyrosine peptide binding and characterisation of slow conformational exchange processes, Journal of Molecular Biology/JMB Online 299(3): 771-788.
- Siegal G.D., Duynhoven J. van & Baldus M. (1999), Biomolecular NMR: recent advances in liquids, solids and screening, Current Opinion in Chemical Biology 3(5): 530-536.
- Kolczak U., Salgado J., Siegal G.D., Saraste M. & Canters G.W. (1999), Paramagnetic NMR studies of blue and purple copper proteins, Biospectroscopy 5(S5): s19-s32.
- Hoedemaeker F.J., Siegal G.D., Roe S.M., Driscoll P.C. & Abrahams J.P. (1999), Crystal structure of the C-terminal SH2 domain of the p85a regulatory subunit of phosphoinositide 3-kinase: a SH2 domain mimicking its own substrate, Journal of Molecular Biology/JMB Online 292(4): 763-770.
- Pérez J.M.J., Siegal G.D., Kriek J., Hard K., Dijk J., Canters G.W. & Möller W. (1999), The solution structure of the guanine nucleotide exchange domain of human elongation factor 1beta reveals a striking resemblance to that of EF-Ts from Escherichia coli, Structure Fold Design 7(2): 217-226.
- Siegal G.D. (1999), The surprisingly flexible PTB domain, Nature Structural Biology 6: 7-10.
- Siegal G.D., Davis B., Kristensen S., Sankar A., Panayotou G., Linacre J., Stein R.R., Waterfield M.D. & Driscoll P.C. (1998), Solution structure of the C-terminal SH2 domain of the p85alpha regulatory subunit of phosphoinositide 3-kinase, Journal of Molecular Biology/JMB Online 276: 461-478.
- Pérez J.M.J., Kriek J., Dijk J., Möller W., Siegal G.D., Hard K., Kalverda A.P. & Canters G.W. (1998), 1H, 15N and 13C chemical shift assignment of the guanine nucleotide exchange domain of human elongation factor-one beta, Journal of Biomolecular NMR 12: 467-468.
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