Anne Wentink
Assistant Professor
- Name
- Dr. A.S. Wentink
- Telephone
- +31 71 527 4534
- a.s.wentink@lic.leidenuniv.nl
The research of Anne Wentink is focused on understanding the role of chaperone mediated disaggregation of protein aggregates in the context of neurodegenerative disease.
More information about Anne Wentink
PhD candidates
News
-
How cells determine the fate of proteins (and can we do it too?) -
Gravitation funding for five projects with Leiden researchers -
Can Parkinson's be stopped by unravelling protein fibres? Anne Wentink finds out with a Vidi grant from NWO -
Vidi grants for eight researchers from Leiden University -
Leiden University strengthens its focus on Chemical Biology and Medicinal Chemistry with seven new group leaders
Biography
Dr. Anne S Wentink (09-05-1990) studied Biochemistry at University College London (UCL) from 2007-2010. She obtained a BBSRC scholarship to pursue a PhD in the lab of Prof John Christodoulou at UCL (2010-2015). Here she used solution-state NMR spectroscopy to study the structure and dynamics of the ribosome associated bacterial chaperone Trigger Factor and its interaction with nascent polypeptide chains.
Her postdoctoral research with Prof Bernd Bukau at Heidelberg University (since 2015) on the molecular mechanism of amyloid fibre disaggregation by the human Hsp70 chaperone cumulated in two back-to-back publications in Nature in 2020. In January 2022, she started her own research group as assistant professor within the division of Macromolecular Biochemistry focused on understanding the role of chaperone mediated disaggregation of protein aggregates in the context of neurodegenerative disease.
Curriculum Vitae
Personal information
Nationality: Dutch
Date of birth: 9 May 1990
Education
2015 PhD in Structural and Molecular Biology, University College London (UCL), United Kingdom. Thesis title: “Structure and dynamics of the bacterial chaperone Trigger Factor”
Advisor: Prof. J. Christodoulou
Current and previous research positions
2022 – now Assistant Professor, Leiden Institute of Chemistry, Leiden University, The Netherlands
2015 – 2021 Postdoctoral Researcher, Centre for Molecular Biology of Heidelberg University and German Cancer Research Centre, Germany
Grants, awards, recognitions
2023 NWO Vidi Grant (k€ 800)
Contribution to teaching and supervision
- Course for MSc Life Science & Technology and Chemistry at Leiden University: Protein Folding, Misfolding and Design
- Course for BSc Life Science & Technology at Leiden University: Life Sciences
- Supervisor of BSc and MSc research projects and colloquia
(Co-) Supervision of 5 PhD students since 2019.
Other responsibilities
- Member of Programme Committee BSc Life Science & Technology
- Member of LUF International Study Fund (LISF) Committee
- Member of Organising Committee LED3 seminar series
- Organiser of Leiden Proteostasis Community meetings
- Member of Participant Council of the Dutch NMR Discussion Group
Publication record including recent representative publications
13 papers with >650 citations, H-index 11 (Web of Science)
Full publication record: Leiden University
- Beton et al., Cooperative Amyloid Fibre Binding and Disassembly by the HSP70 disaggregase, EMBO J, 2022;41(16). DOI: 10.15252/embj.2021110410
- Wentink et al., Molecular dissection of amyloid disaggregation by the human Hsp70 chaperone, Nature, 2020;587(7834):483-488. DOI: 10.1038/s41586-020-2904-6
- Faust et al., Hsp40s employ class-specific regulation to drive Hsp70 functional diversity, Nature, 2020;587(7834) :489-494. DOI: 10.1038/s41586-020-2906-4
- Nachman et al., Disassembly of Tau fibrils by the human Hsp70 disaggregation machinery liberates small seeding-competent species. JBC 2020. DOI: 10.1074/jbc.RA120.013478
Presentations including at international conferences
~15 invited presentations at universities and institutes, and oral communications at international conferences
Assistant Professor
- Science
- Leiden Institute of Chemistry
- LIC/Chemical Biology
- LIC/CB/Macromolecular Biochemistry
- Herling T.W., Cassaignau A.M.E., Wentink A.S., Peter Q.A.E., Kumar P.C., Kartanas T., Schneider M.M., Cabrita L. D., Christodoulou J. & Knowles T.P.J. (2024), Thermodynamic profiles for cotranslational trigger factor substrate recognition, Science Advances 10(28): eadn4824.
- Zoltsman G., Dang T.L., Kuchersky M., Faust O., Silva M.S., Ilani T., Wentink A.S., Bukau B. & Rosenzweig R. (2024), A unique chaperoning mechanism in class A JDPs recognizes and stabilizes mutant p53, Molecular Cell 84(8): 1512-1526.
- Wentink A.S. & Rosenzweig R. (2023), Protein disaggregation machineries in the human cytosol, Current Opinion in Structural Biology 83: 102735.
- Beton J.G., Monistrol J., Wentink A.S., Johnston E.C., Roberts A.J., Bukau B.G., Hoogenboom B.W. & Saibil H.R. (2022), Cooperative amyloid fibre binding and disassembly by the Hsp70 disaggregase, The EMBO Journal 41(16): e110410.
- Nachman E., Wentink A.S., Madiona K., Bousset L., Katsinelos T., Allinson K., Kampinga H., McEwan W.A., Jahn T.R., Melki R., Mogk A., Bukau B. & Nussbaum-Krammer C. (2020), Disassembly of Tau fibrils by the human Hsp70 disaggregation machinery generates small seeding-competent species, Journal of Biological Chemistry 295(28): 9676-9690.
- Serlidaki D., Waarde M.A.W.H. van, Rohland L., Wentink A.S., Dekker S.L., Kamphuis M.J., Boertien J.M., Brunsting J.F., Nillegoda N.B., Bukau B., Mayer M.P., Kampinga H.H. & Bergink S. (2020), Functional diversity between HSP70 paralogs caused by variable interactions with specific co-chaperones, Journal of Biological Chemistry 295(21): 7301-7316.
- Faust O., Abayev-Avraham M., Wentink A.S., Maurer M., Nillegoda N.B., London N., Bukau B. & Rosenzweig R. (2020), HSP40 proteins use class-specific regulation to drive HSP70 functional diversity, Nature 587(7834): 489-+.
- Wentink A.S., Nillegoda N.B., Feufel J., Ubartaite G., Schneider C.P., De Los Rios P., Hennig J., Barducci A. & Bukau B. (2020), Molecular dissection of amyloid disaggregation by human HSP70, Nature 587(7834): 483-+.
- Mattos E.P. de, Wentink A., Nussbaum-Krammer C., Hansen C., Bergink S., Melki R. & Kampinga H.H. (2020), Protein Quality Control Pathways at the Crossroad of Synucleinopathies, Journal of Parkinson's Disease 10(2): 369-382.
- Wentink A., Nussbaum-Krammer C. & Bukau B. (2019), Modulation of Amyloid States by Molecular Chaperones, Cold Spring Harbor Perspectives in Biology 11(7): a033969.
- Nillegoda N.B., Wentink A.S. & Bukau B. (2018), Protein Disaggregation in Multicellular Organisms, Trends in Biochemical Sciences 43(4): 285-300.
- Waudby C.A., Wlodarski T., Karyadi M.E., Cassaignau A.M.E., Chan S.H.S., Wentink A.S., Schmidt-Engler J.M., Camilloni C., Vendruscolo M., Cabrita L.D. & Christodoulou J. (2018), Systematic mapping of free energy landscapes of a growing filamin domain during biosynthesis, Proceedings of the National Academy of Sciences 115(39): 9744-9749.
- Mashaghi A., Bezrukavnikov S., Minde D.P., Wentink A.S., Kityk R., Zachmann-Brand B., Mayer M.P., Kramer G., Bukau B. & Tans S.J. (2016), Alternative modes of client binding enable functional plasticity of Hsp70, Nature 539(7629): 448-451.
- Cabrita L.D., Cassaignau A.M.E., Launay H.M.M., Waudby C.A., Wlodarski T., Camilloni C., Karyadi M.E., Robertson A.L., Wang X.L., Wentink A.S., Goodsell L.S., Woolhead C.A., Vendruscolo M., Dobson C.M. & Christodoulou J. (2016), A structural ensemble of a ribosome-nascent chain complex during cotranslational protein folding, Nature Structural and Molecular Biology 23(4): 278-285.
- Deckert A., Waudby C.A., Wlodarski T., Wentink A.S., Wang X.L., Kirkpatrick J.P., Paton J.F.S., Camilloni C., Kukic P., Dobson C.M., Vendruscolo M., Cabrita L.D. & Christodoulou J. (2016), Structural characterization of the interaction of alpha-synuclein nascent chains with the ribosomal surface and trigger factor, Proceedings of the National Academy of Sciences 113(18): 5012-5017.