Dissertation
The advantages and disadvantages of bioorthogonal proteins
This thesis describes the use of bioorthogonal proteins in immunological settings. It provides an introduction towards the field of protein modification, which was used throughout the thesis for the expression of proteins containing unnatural amino acids.
- Author
- Groenewold, G.J.M.
- Date
- 17 February 2021
- Links
- Thesis in Leiden University Scholarly Publications
This thesis describes the use of bioorthogonal proteins in immunological settings. It provides an introduction towards the field of protein modification, which was used throughout the thesis for the expression of proteins containing unnatural amino acids. It challenges this protein expression and the subsequent purification. With this successfully challenged, the use of the protein was assessed in antigen activation studies. It was analyzed whether the unnatural amino acids were tolerated by the T cell receptor and the ligation handles of the unnatural amino acids were subsequently used for visualization purposes. Furthermore, this thesis describes the possible use of azido HRP as a new tool for immunohistology.