Universiteit Leiden

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Dissertation

Essentiality of conserved amino acid residues in β-lactamase

Evolution acts via mutations in amino acid sequences. Substitution of essential amino acids leads to a nonfunctional protein. l.

Author
Chikunova, A.
Date
31 May 2022
Links
Thesis in Leiden Repository

Thus, the number of essential residues is limited by evolutionary pressure. The roles of all non-catalytic essential residues in class A β-lactamases are described with a large-scale experiments, as well as specific functions of a few residues. The results show that residues close to the active site and farther away have different reasons for being essentia

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