Marije Niemeijer
Assistant professor
- Name
- Dr. M.C. Niemeijer
- Telephone
- +31 71 527 6105
- m.c.niemeijer@lacdr.leidenuniv.nl
- ORCID iD
- 0000-0003-0045-2317
Dr. Marije Niemeijer is Assistant Professor at the Drug Discovery & Safety division. She received her Masters degree cum laude in Bio-pharmaceutical Sciences with the specialization Toxicology at Leiden University. Thereafter, she did her PhD at the department of Toxicology of LACDR at Leiden University. Here, she studied the regulation of ER stress signalling upon chemical exposure by combining siRNA screening, high-content imaging and transcriptomic approaches. As a post-doctoral researcher, she focused on the application of 3D liver models for toxicity testing. Now as assistant professor, she aims to dissect molecular mechanisms of drug action in both the context of drug efficacy as well as toxicity using advanced in vitro test systems that are fit for this purpose. To improve the prediction of adverse drug reactions, it is key to identify the molecular-initiation-event and understand the mechanism leading to adverse events. Since a single test system cannot predict all, it is essential to identify a drug safety testing strategy using a specific battery of test systems both encompassing more simple high-throughput and advanced systems. Through omics approaches, affected genes or networks for different areas within the chemical space or for specific pathologies can be identified and used as reporter genes or as drug targets. To further refine chemical risk assessment, she aims to characterize the inter-individual variability in the activation of these critical gene networks upon chemical exposure.
Profile
Projects:
- RISK-HUNT3R (RISK assessment of chemicals integrating HUman centric Next generation Testing strategies promoting the 3Rs)
- PARC (Partnership for the Assessment of Risks from Chemicals)
- TD-TRAQ (Characterisation of human variability in toxicodynamics: towards the development of quantitative Adverse Outcome Pathways, qAOPs)
- TXG-MAP (Translational quantitative Toxicogenomics mechanism-based AOPmapping for human NAM-based risk assessment)
Courses:
- BSc BFW2 Toxicologie
- BSc BFW2 practicum Cellulair Biochemie
Assistant professor
- Science
- Leiden Academic Centre for Drug Research
- LACDR/Toxicology
- Niemeijer M.C., Więcek W., Fu S., Huppelschoten S., Bouwman R.J.P., Baze A., Parmentier C., Richert L., Paules R.S., Bois F.Y. & Water B. van de (2024), Mapping interindividual variability of toxicodynamics using high-throughput transcriptomics and primary human hepatocytes from fifty donors, Environmental Health Perspectives 132(3): 37005.
- Danilyuk T.Y., Niemeijer M.C., Wijaya L.S., Snijders K., Berk L. van der, Braak S.J. ter, Callegaro G., Bouwman R.J.P., Le Dévédec S.E. & Water B. van de (2023), Human-induced pluripotent stem cell reporters for high-content screening of stress response activation identifying target organ-specific toxicities, Toxicology Letters 384: S192.
- Niemeijer M.C. (7 February 2023), Unfolding the regulation of stress response pathways upon liver injury (Dissertatie. Leiden Academic Centre for Drug Research (LACDR), Faculty of Science, Leiden University). Supervisor(s): Water B. van de.
- Braak S.J. ter, Niemeijer M.C., Wolters L., Le Dévédec S.E., Bouwman R.J.P. & Water B. van de (2022), Towards an advanced testing strategy for genotoxicity using image-based 2D and 3D HepG2 DNA damage response fluorescent protein reporters, Mutagenesis 37(2): 130-142.
- Heldring M.M., Wijaya L.S., Niemeijer M.C., Yang H., Lakhal T., Le Dévédec S.E., Water B. van de & Beltman J.B. (2022), Model-based translation of DNA damage signaling dynamics across cell types, PLoS Computational Biology 18(7): e1010264.
- Llewellyn S.V., Kermanizadeh A., Ude V., Jacobsen N.R., Conway G.E., Shah U.K., Niemeijer M.C., Moné M.J., Water B. van de, Roy S., Moritz W., Stone V., Jenkins G.J.S. & Doak S.H. (2022), Assessing the transferability and reproducibility of 3D in vitro liver models from primary human multi-cellular microtissues to cell-line based HepG2 spheroids, Toxicology In Vitro 85: 105473.
- Sharma R.P., Wolters L., Braak B.T., Niemeijer M., Sparham C., Nicol B., White A., Water B. van de, Middleton A.M. & Beltman J.B. (2022), Deciphering the quantitative relationship between chemical kinetics and Nrf2 pathway dynamics, Toxicology Letters 368(Supplement 1): S288.
- Wijaya L.S., Trairatphisan P., Gabor A., Niemeijer M.C., Keet J., Alcalà Morera A., Snijders K.E., Wink S., Yang H., Schildknecht S., Stevens J.L., Bouwman R.J.P., Kamp H., Hengstler J., Beltman J.B., Leist M., Le Dévédec S.E., Saez-Rodriguez J. & Water B. van de (2021), Integration of temporal single cell cellular stress response activity with logic-ODE modeling reveals activation of ATF4-CHOP axis as a critical predictor of drug-induced liver injury, Biochemical Pharmacology 190: 114591.
- Llewellyn S.V., Niemeijer M., Nymark P., Moné M.J., Water B.van de, Conway G.E., Jenkins G.J.S. & Doak S.H. (2021), In vitro three-dimensional liver models for nanomaterial DNA damage assessment, Small 17(15): e2006055.
- Braak B. ter, Niemeijer M., Boon R., Parmentier C., Baze A., Richert. L., Huppelschoten S., Wink S., Verfaillie C. & Water B. van de (2021), Systematic transcriptome-based comparison of cellular adaptive stress response activation networks in hepatic stem cell-derived progeny and primary human hepatocytes, Toxicology In Vitro 73: 105107.
- Snijders K.E., Fehér A., Táncos Z., Bock I., Téglási A., Berk L. van den, Niemeijer M., Bouwman P., Le Dévédec S.E., Moné M.J., Rossom R. van, Kumar M., Wilmes A., Jennings P., Verfaillie C.M., Kobolák J., Braak B. ter, Dinnyés A. & Water B. van de (2021), Fluorescent tagging of endogenous heme oxygenase-1 in human induced pluripotent stem cells for high content imaging of oxidative stress in various differentiated lineages, Archives of Toxicology 95(10): 3285-3302.
- Callegaro G., Kunnen S.J., Trairatphisan P., Grosdidier S., Niemeijer M., Hollander W. den, Guney E., Piñero Gonzalez J., Furlong L., Webster Y.W., Saez-Rodriguez J., Sutherland J.J., Mollon J., Stevens J.L. & Water B. van de (2021), The human hepatocyte TXG-MAPr: gene co-expression network modules to support mechanism-based risk assessment, Archives of Toxicology 95(12): 3745-3775.
- Yang H., Niemeijer M.C., Water B. van de & Beltman J.B. (2020), ATF6 Is a Critical Determinant of CHOP Dynamics during the Unfolded Protein Response, iScience 23(2): 100860.
- Mav. D., Phadke D.P., Balik-Meisner M.R., Merrick B.A., Auerbach S., Niemeijer M.C., Huppelschoten S., Baze A., Parmentier C., Richert L., Water B. van de, Shah R.R. & Paules R.S. (2020), Utility of extrapolating human S1500+ genes to the whole transcriptome: tunicamycin case study, Bioinformatics and Biology Insights 14: 1-14.
- Schimming J.P., Braak B. ter, Niemeijer M., Wink S. & Water B. van de (2019), System Microscopy of Stress Response Pathways in Cholestasis Research. In: Vinken M. (Ed.), Experimental Cholestasis Research. Methods in Molecular Biology no. 1981. New York, NY, U.S.A.: Humana Press. 187-202.
- Niemeijer M.C., Hiemstra S.W., Wink S., Hollander W. den, Braak S.J. ter & Water B. van de (2018), Systems microscopy approaches in unraveling and predicting Drug-Induced Liver Injury (DILI). In: Chen M. & Will Y. (Eds.), Drug-Induced Liver Toxicity. Methods in Pharmacology and Toxicology. New York: Humana Press. 611-625.
- Hiemstra S., Niemeijer M., Koedoot E., Wink S., Pip J.E., Vlasveld M., Zeeuw E. de , Os B. van, White A. & Water B. van de (2017), Comprehensive Landscape of Nrf2 and p53 Pathway Activation Dynamics by Oxidative Stress and DNA Damage, Chemical Research in Toxicology 30(4): 923-933.
- Wink S., Hiemstra S., Huppelschoten S., Danen E.H.J., Niemeijer M., Hendriks G., Vrieling H., Herpers B. & Water B. van de (2014), Quantitative high content imaging of cellular adaptive stress response pathways in toxicity for chemical safety assessment, Chemical Research in Toxicology 27(3): 338-355.