More information about Bobby Florea

Curriculum Vitae

Personal information

Nationality:   Dutch
Date of birth:   14 October 1973

Education

2003   PhD, Leiden Amsterdam Center for Drug Research, Leiden University, The Netherlands. Thesis title: “Polymeric carriers for drug and gene delivery”
Promotors: Prof. G. Borchard and Prof. H.E. Junginger
1998   MSc in Bio Pharmaceutical Sciences (BFW), Leiden Amsterdam Centre for Drug Research, Leiden University, The Netherlands
1995   Propedeuse in Chemistry, Leiden Institute for Chemistry, Leiden University, The Netherlands

Current and previous research positions

2022 – now    Assistant Professor and Executive Officer Chemical Proteomics Reagens, Leiden Institute for Chemistry, Leiden University, The Netherlands
2010 – 2022   Scientist and Executive Officer Chemical Proteomics Reagens, Leiden Institute of Chemistry, Leiden University, The Netherlands
2004 – 2010   Postdoctoral fellow, Leiden Institute for Chemistry, Leiden University, The Netherlands (Prof. H.S. Overkleeft)
2002 – 2004   Postdoctoral fellow, Cell Biology and Genetics Erasmus Medical Center, Erasmus University (Prof. J.H.J Hoeijmakers)
1997 – 1998   Student assistant, Ciba Geigy (Novartis), Basel, Switzerland (Prof. T. Arvinte)

Contribution to teaching and supervision

• Chemical Analysis Methods course for 1st year BSc Molecular Science and Technology at Leiden University: 
• Guest lecturer
• 2012 – 2018      Chemical Genetics master class (Prof. J. Brouwer)
• 2013 – 2019     Honour class (Prof. M. Noteborn) 
• 2014 – 2017     Netherlands Research School Chemical Biology (Prof. J. Brouwer)

Co-promoter of 3 PhD theses, co-supervision 9 PhD students,  supervision of 22 MSc and 71 BSc students  since 2004.

Other current responsibilities since 2016

• Editor of the book: Activity-Based Proteomics, Methods in Biology, 2017, vol 1491, editors HS Overkleeft and BI Florea, Humana New York, NY. DOI: 10.1007/978-1-4939-6439-0
• Organizer and Chair of the 2017 European Activity based proteomics meeting in Leiden
• Reviewer for 21 international journals and 3 European funding agencies 
• Executive Officer Chemical Proteomics Reagents
• Setup and daily operation of the high-resolution mass spectrometry expertise lab of the Leiden Institute for Chemistry, Leiden University
• Setup of protein analysis and quantification methods and training of young researchers in these
• Co-supervisor and co-manager of the Dutch Compound Library repository of the Leiden Institute for Chemistry, Leiden University

Publication record including five recent representative publications

115 publications with 2600 citations, H-index 37 (Web of Science)

Full publication record: Leiden University

• Besse L, et al., Florea BI, Overkleeft HS, Driessen C, Treatment with HIV-protease inhibitor Nelfinavir identifies membrane lipid composition & fluidity as therapeutic target in advanced multiple myeloma. Cancer Res. 2021, 81(17): 4581-4593. DOI: 10.1158/0008-5472.CAN-20-3323
• Inagawa T, et al., Florea BI, Verkaik NS, van Gent DC, C-terminal extensions of Ku70 and Ku80 differentially influence DNA end binding properties, Int. J. Molec. Sci. 2020, 21(18): 6725. DOI: 10.3390/ijms21186725
• Armstrong Z, et al., Florea BI, Overkleeft HS, Davies GJ, Manno-epi-cyclophellitols enable activity-based protein profiling of human α-mannosidases and discovery of new golgi mannosidase II inhibitors, J. A. C. S. 2020, 142(30): 13021-13029. DOI: 10.1021/jacs.0c03880
• Rooden EJ, Florea BI, et al., Aerts JMFG, van der Stelt M, Chemical proteomic analysis of serine hydrolase activity in Niemann-Pick type C mouse brain, Front. Neurosci. 2018, 12: 440. DOI: 10.3389/fnins.2018.00440
• de Bruin G. de, Xin BT, Florea BI, Overkleeft HS, Proteasome subunit selective activity-based probes report on proteasome core particle composition in a native polyacrylamide gel electrophoresis fluorescence-resonance energy transfer assay, J. A. C. S. 2016, 138(31): 9874-9880. DOI: 10.1021/jacs.6b04207

Publications