Anton Raap
Professor Molecular Cytology
- Name
- Prof.dr. A.K. Raap
- Telephone
- +31 71 526 9263
- a.k.raap@lumc.nl
- ORCID iD
- 0000-0003-1483-7816
For more information please visit the LUMC website
One can read the fascination from my face when it comes to imaging and concepts in cell biology and genetics. In the actual image formation, microscopy and the molecular sciences take a central position: with a number of elegant experimental procedures, the biomolecular processes in cell and chromosomes are projected on the retina in colourful images. The brain-eye interaction then takes over. The images are interpreted and put in perspective of cell biological and genetic hypotheses. Usually this process inspires to further experimentation so as to get the sharper picture of the truth of the healthy and diseased cell.
There are a few other aspects in this work that contribute to my fascination. The step to diagnostic application is often a small one, the actual images are esthetically pleasing and it is a joy to teach the images and concepts.
The title of his inaugural lecture was 'Cellen en moleculen microscopisch in beeld'.
Professor Molecular Cytology
- Faculteit Geneeskunde
- Divisie 4
- Divisie 4
- Janssen GMC, Schwertman P, Wanga TAT, Tafrechi RSJ, van den Broek PJA & Raap AK (2009), Transient and constitutive repression of cytoplasmic translation signaling in cells with mtDNA mutation, Cellular and Molecular Life Sciences 66(4): 721-730.
- Janssen GMC, Hensbergen PJ, van Bussel FJ, Balog CIA, Maassen JA, Deelder AM & Raap AK (2007), The A3243G tRNA(Leu(UUR)) mutation induces mitochondrial dysfunction and variable disease expression without dominant negative acting translational defects in complex IV subunits at UUR codons, Human Molecular Genetics 16(20).
- Tafrechi RSJ, van de Rijke FM, Allallou A, Larsson C, Sloos WCR, van de Sande M, Wahlby C, Janssen GMC & Raap AK (2007), Single-cell A3243G mitochondrial DNA mutation load assays for segregation analysis, Journal of Histochemistry and Cytochemistry 55(11).
- Raap AK & Tanke HJ (2006), COmbined Binary RAtio fluorescence in situ hybridiziation (COBRA-FISH): development and applications, Cytogenetic and Genome Research 114(3-4).
- Maassen JA, Tafrechi RSJ, Janssen GMC, Raap AK, Lemkes HH & 't Hart LM (2006), New insights in the molecular pathogenesis of the maternally inherited diabetes and deafness syndrome, Endocrinology and Metabolism Clinics of North America 35(2).
- Dijkman R, Tensen CP, Jordanova ES, Knijnenburg J, Hoefnagel JJ, Mulder AA, Rosenberg C, Raap AK, Willemze R, Szuhai K & Vermeer MH (2006), Array-based comparative genomic hybridization analysis reveals recurrent chromosomal alterations and prognostic parameters in primary cutaneous large B-cell lymphoma., Journal of Clinical Oncology 24(2): 296-305.
- Dijkman R, Tensen CP, Jordanova ES, Knijnenburg J, Hoefnagel JJ, Mulder AA, Rosenberg C, Raap AK, Willemze R, Szuhai KR & Vermeer MH (2006), Array-based comparative genomic hybridization analysis reveals recurrent chromosomal alterations and prognostic parameters in primary cutaneous large B-cell lymphoma, Journal of Clinical Oncology 24(2).
- Raap AK & Tanke HJ (2006), COmbined Binary RAtio fluorescence in situ hybridiziation (COBRA-FISH): development and applications., Cytogenetic and Genome Research 114(3-4): 222-6.
- Vries RGJ, Bezrookove V, Zuijderduijn LMP, Kia SK, Houweling A, Oruetxebarria I, Raap AK & Verrijzer CP (2005), Cancer-associated mutations in chromatin remodeler hSNF5 promote chromosomal instability by compromising the mitotic checkpoint, Genes and Development 19(6).
- Tafrechi RSJ, Svensson PJ, Janssen GMC, Szuhai K, Maassen JA & Raap AK (2005), Distinct nuclear gene expression profiles in cells with mtDNA depletion and homoplasmic A3243G mutation, Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis 578(1-2).
- Guikema JEJ, Fenton JAL, de Boer C, Kleiverda K, Brink AATP, Raap AK, Estrov Z, Schuuring E & Kluin PM (2005), Complex biallelic IGH rearrangements in IgM-expressing Z-138 cell line: Involvement of downstream immunoglobulin class switch recombination, Genes, Chromosomes and Cancer 42(2).
- Tong, M.; Jacobi, C.E.; Rijke, F.M. van de; Kuijper, S.; Werken, S. van de; Lowary, T.L.; Hokke, C.H.; Appelmelk, B.J.; Nagelkerke, N.J.D.; Tanke, H.J.; Gijlswijkh, R.P.M. van; Veuskens, J.; Kolk, A.H.J. & Raap, A.K. (2005), A multiplexed and miniaturized serological tuberculosis assay identifies antigens that discriminate maximally between TB and non-TB sera., Journal of Immunological Methods 301(1-2): 154-63.
- Tong M, Jacobi CE, van de Rijke FM, Kuijper S, van de Werken S, Lowary TL, Hokke CH, Appelmelk BJ, Nagelkerke NJD, Tanke HJ, van Gijlswijkh RPM, Veuskens J, Kolk AHJ & Raap AK (2005), A multiplexed and miniaturized serological tuberculosis assay identifies antigens that discriminate maximally between TB and non-TB sera, Journal of Immunological Methods 301(1-2).
- Wu Y, de Kievit P, Vahlkamp L, Pijnenburg D, Smit M, Dankers M, Melchers D, Stax M, Boender PJ, Ingham C, Bastiaensen N, de Wijn R, van Alewijk D, van Damme H, Raap AK, Chan AB & van Beuningen R (2004), Quantitative assessment of a novel flow-through porous microarray for the rapid analysis of gene expression profiles, Nucleic Acids Research 32(15).
- Bezrookove V, van Zelderen-Bhola SL, Brink A, Szuhai K, Raap AK, Barge R, Beverstock GC & Rosenberg C (2004), A novel t(6;l4)(q25 similar to q27;q32) in acute myelocytic leukemia involves the BCL11B gene, Cancer Genetics and Cytogenetics 149(1).
- Wiegant J, Brouwer K, Bezrookove V, Smits S, Raap AK, Bout A & Tanke HJ (2004), Molecular cytogenetic characterization by combined COBRA-FISH and fiber-FISH: application to PER.C6 (R) cells, BioTechniques 37(2).
- Ferber MJ, Eilers P, Schuuring E, Fenton JAL, Fleuren GJ, Kenter G, Szuhai K, Smith DI, Raap AK & Brink AATP (2004), Positioning of cervical carcinoma and Burkitt lymphoma translocation breakpoints with respect to the human papillomavirus integration cluster in FRA8C at 8q24.13, Cancer Genetics and Cytogenetics 154(1).
- Maassen JA, 't Hart LM, van Essen E, Heine RJ, Nijpels G, Tafrechi RSJ, Raap AK, Janssen GMC & Lemkes HHPJ (2004), Mitochondrial diabetes - Molecular mechanisms and clinical presentation, Diabetes 53.
- Raap AK, van der Burg MJM, Knijnenburg J, Meershoek E, Rosenberg C, Gray JW, Wiegant J, Hodgson JG & Tanke HJ (2004), Array comparative genomic hybridization with cyanin cis-platinum-labeled DNAs, BioTechniques 37(1).
- Heetebrij RJ, Talman EG, Von Velzen MA, Von Gijlswijk RPM, Snoeijers SS, Schalk M, Wiegant J, Von der Rijke F, Kerkhoven RM, Raap AK, Tanke HJ, Reedijk J & Houthoff HJ (2003), Platinum(II)-based coordination compounds as nucleic acid labeling reagents: Synthesis, reactivity, and applications in hybridization assays, ChemBioChem 4(7).
- Engels H, Ehrbrecht A, Zahn S, Bosse K, Vrolijk H, White S, Kalscheuer V, Hoovers JMN, Schwanitz G, Propping P, Tanke HJ, Wiegant J & Raap AK (2003), Comprehensive analysis of human subtelomeres with combined binary ratio labelling fluorescence in situ hybridisation, European Journal of Human Genetics 11(9).
- Bezrookove V, Smits R, Moeslein G, Fodde R, Tanke HJ, Raap AK & Darroudi F (2003), Premature chromosome condensation revisited: A novel chemical approach permits efficient cytogenetic analysis of cancers, Genes, Chromosomes and Cancer 38(2).
- Hagedoorn R, Joseph R, Kasanmoentalib S, Eilers P, Killian J & Raap AK (2003), Chemical RNA Labeling without 3 ' end bias using fluorescent cis-platin compounds, BioTechniques 34(5).
- van de Rijke FM, Heetebrij RJ, Talman EG, Tanke HJ & Raap AK (2003), Fluorescence properties, thermal duplex stability, and kinetics of formation of cyanin platinum DNAs, Analytical Biochemistry 321(1).
- Frei K, Szuhai K, Lucas T, Weipoltshammer K, Schofer C, Ramsebner R, Baumgartner WD, Raap AK, Bittner R, Wachtler FJ & Kirschhofer K (2002), Connexin 26 mutations in cases of sensorineural deafness in eastern Austria, European Journal of Human Genetics 10(7).
- Nilsson M, Gullberg M, Dahl F, Szuhai K & Raap AK (2002), Real-time monitoring of rolling-circles amplification using a modified molecular beacon design.
- Brink AATP, Wiegant JCAG, Szuhai K, Tanke HJ, Kenter GG, Fleuren GJ, Schuuring E & Raap AK (2002), Simultaneous mapping of human papillomavirus integration sites and molecular karyotyping in short-term cultures of cervical carcinomas by using 49-color combined binary ratio labeling fluorescence in situ hybridization, Cancer Genetics and Cytogenetics 134(2).
- Gevers EF, Van Der Eerden BCJ, Karperien M, Raap AK, Robinson ICAF & Wit JM (2002), Localization and regulation of the growth hormone receptor and growth hormone-binding protein in the rat growth plate, Journal of Bone and Mineral Research 17(8).
- Darroudi F, Bezrookove V, Fomina J, Mesker WE, Wiegant JCAG, Raap AK & Tanke HJ (2002), Insights into the sites of X ray and neutron induced chromosomal aberrations in human lymphocytes using COBRA-MFISH, Radiation Protection Dosimetry 99(1-4).
- van de Rijke F, Zijlmans H, Li S, Vail T, Raap AK, Niedbala RS & Tanke HJ (2001), Up-converting phosphor reporters for nucleic acid microarrays, Nature Biotechnology 19(3).
- Szuhai K, Sandhaus E, Kolkman-Uljee SM, Lemaitre M, Truffert JC, Dirks RW, Tanke HJ, Fleuren GJ, Schuuring E & Raap AK (2001), A novel strategy for human papillomavirus detection and genotyping with SybrGreen and molecular beacon polymerase chain reaction, American Journal of Pathology 159(5).
- van de Rijke FM, Florijn RJ, Tanke HJ & Raap AK (2000), DNA fiber-FISH staining mechanism.
- Szuhai K, Bezrookove V, Wiegant J, Vrolijk J, Dirks RW, Rosenberg C, Raap AK & Tanke HJ (2000), Simultaneous molecular karyotyping and mapping of viral DNA integration sites by 25-color COBRA-FISH.
- van den Ouweland JMW, de Klerk JBC, van de Corput MP, Dirks RW, Raap AK, Scholte HR, Huijmans JGM, Hart LMT, Bruining GJ & Maassen JA (2000), Characterization of a novel mitochondrial DNA deletion in a patient with a variant of the Pearson marrow-pancreas syndrome.
- Bezrookove V, Hansson K, van der Burg M, van der Smagt JJ, Hilhorst-Hofstee Y, Wiegant J, Beverstock GC, Raap AK, Tanke H, Breuning MH & Rosenberg C (2000), Individuals with abnormal phenotype and normal G-banding karyotype: improvement and limitations in the diagnosis by the use of 24-colour FISH.
- Wiegant J, Bezrookove V, Rosenberg C, Tanke HJ, Raap AK, Zhang HG, Bittner M, Trent JM & Meltzer P (2000), Differentially painting human chromosome arms with combined binary ratio-labeling fluorescence in situ hybridization.
- van Gijlswijk RPM, van de Corput MPC, Bezrookove V, Wiegant J, Tanke HJ & Raap AK (2000), Synthesis and purification of horseradish peroxidase-labeled oligonucleotides for tyramide-based fluorescence in situ hybridization.
- Wiegant JCAG, van Gijlswijk RPM, Heetebrij RJ, Bezrookove V, Raap AK & Tanke HJ (1999), ULS: a versatile method of labeling nucleic acids for FISH based on a monofunctional reaction of cisplatin derivatives with guanine moieties.
- Rosenberg C, Schut TB, Mostert M, Tanke H, Raap A, Oesterhuis JW & Looijenga L (1999), Chromosomal gains and losses in testicular germ cell tumors of adolescents and adults investigated by a modified comparative genomic hybridization approach.