Research project
Efficient gene-targeting during meiosis
Efficient gene-targeting during meiosis
- Duration
- 2013 - 2017
- Contact
- Sylvia de Pater
- Funding
-
Partnership STW RijkZwaan project 12428
Using CRISPR/Cas9, DSB are induced in target sequences of the cruciferin 3 gene (A) or the protoporphyrinogen oxidase gene (B) for site-specific mutagenesis. Plants expressing these nucleases can be transformed with homologous repair templates to obtain specific mutations via homologous recombination (gene-targeting).
- Shen H, Strunks GD, Klemann BJPM, Hooykaas PJJ, de Pater S (2017) CRISPR/Cas9-induced double-strand break repair in Arabidopsis nonhomologous end-joining mutants. doi: 10.1534/g3.116.035204