Publication

Novel Activity-Based Probes for Broad-Spectrum Profiling of Retaining β-Exoglucosidases In Situ and In Vivo

A high-end label: Cyclophellitol aziridine-type activity-based probes allow for ultra-sensitive visualization of mammalian β-glucosidases (GBA1, GBA2, GBA3, and LPH) as well as several non-mammalian β-glucosidases (see picture). These probes offer new ways to study β-exoglucosidases, and configurational isomers of the cyclophellitol aziridine core may give activity-based probes targeting other retaining glycosidase families.

Author

Kallemeijn W.W., Li K.Y., Witte M.D., Marques A.R.A., Aten J., Scheij S., Jiang J.B., Willems L.I., Voorn-Brouwer T.M., Roomen C.P.A.A. van, Ottenhoff R., Boot R.G., Elst H. van den, Walvoort M.T.C., Florea B.I., Codee J.D.C., Marel G.A. van der, Aerts J.M.F.G., Overkleeft H.S.

Date

08 November 2012

Links

DOI link