Lecture
LIC Lecture: 3D Domain Swapping of Proteins: Basics and Recent Developments
- Date
- Wednesday 13 March 2024
- Time
- Location
-
Gorlaeus Building
Einsteinweg 55
2333 CC Leiden - Room
- DM1.19
Abstract
Three-dimensional (3D) domain swapping is a protein oligomerization phenomenon that exchanges the same domain or secondary structural element between molecules. Our research group has shown that various proteins can undergo domain swapping. We found by X-ray crystallographic and spectroscopic analyses that cyt c forms polymers by successive domain swapping, where the C-terminal helix is displaced from its original position in the monomer and cyt c loses its electron transfer function. We have also utilized domain swapping to construct various heme protein assemblies, including nanoring, nanocage, tetrahedron, heterodimer with different active sites, and amyloid fibril. In this lecture, basics and our recent developments of domain swapping will be introduced together with future prospects.
References:
1. S. Hirota, T. Mashima, N. Kobayashi, Chem. Commun., 57, 12074-12086 (2021) (review)
2. S. Sakai, T. Mashima, N. Kobayashi, H. Ogata, L. Duan, R. Fujiki, K. Hengphasatporn, Y. Uda, Y. Shigeta, E. Hifumi, S. Hirota, Nat. Commun., 14, 7807 (2023)